DNAreleasy Advance Direct Lysis Kit

Go From Cells to PCR in 15 Minutes

PCR done the easy way

Are you tired of time consuming extraction processes and costly spin columns when preparing your samples for DNA amplification? With DNAreleasy Advance Direct Lysis Kit, we now have the solution for you.

 

This new cell lysing reagent requires only a 15 minute incubation in a thermal cycler before the DNA is ready for use directly in your PCR – without any further sample processing!

 

DNAreleasy Advance has been successfully used to lyse a wide range of cells. These include saliva, hair roots, animal tissue, plants, drosophila, yeast, bacteria, viruses and many more.

 

Using DNAreleasy Advance is real easy (no pun intended). Just mix cells with 20 μl of the reagent, place in a a thermal cycler or incubator and heat at 75°C for 5 minutes, followed by 96°C for 5 minutes before holding at 20°C for 5 minutes. After the lysis, a part or all of the lysate can be used directly in your PCR or it can be stored at -20°C for future use.

 

DNAreleasy can also be purchased combined with a robust hot-start ready PCR mix with the FastGene Direct PCR Kit.



LITERATURE
User Manual
MSDS
Publications
 
RELATED PRODUCTS

FastGene Direct PCR Kit

BioReady rTaq Polymerase

Mini-Run Gel Electrophoresis  System

Midori Green Nucleic Acid  Staining Solution

Thermal Cyclers

 

 

DNAreleasy Advance results 1
DNAreleasy Advance results 2
Genomic DNA from scallops was isolated with DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield obtained. The same amplified DNA from scallops was used for sequencing resulting in high-quality reads.

 

DNAreleasy Advance results 3
DNAreleasy Advance results 4
Total DNA was isolated from different plant species using DNAreleasy Advance. Melting curves were determined by RT PCR:
Positive control, maize meal, wheat flour, sugar beet, white cabbage, negative control
Genomic DNA was isolated using DNAreleasy Advance and analyzed by qPCR:
(1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melangogaster, (6) horse meat
SPECIFICATIONS
Part Numbers LS06
Amount used in PCR Up to 10%
SYBR Green Compatible Yes
Spectrophotometer Compatible No
Cell and Tissue Compatible Yes
Lysate Can Frozen for Use Later Yes, at -20°C
Storage 1 year at -20°C

 

ORDERING - DNAreleasy Advance , LS06