Fast DNA-spin Plasmid Purification Kit

Watch Your Purity Rise!

Faster and higher yields

The Fast DNA-spin Plasmid Purification Kit employs a simple two-step process – break open walled cells and purify plasmid DNA. Based on the proven technology of optimized alkali lysis buffering system and silica membrane, this kit will isolate extremely pure plasmid DNA from bacteria. Its big advancement over the standard DNA-spin Plasmid Purfication Kit is that it uses a new lysis buffer allowing the adsorption of DNA onto silica membrane in the presence of high salt. Now purify DNA in under 12 minutes (vs 20-30 minutes) with yields that are 20-30% higher.

 

The Fast DNA-spin kit also includes a neutralization buffering technique for gentle pH conditions during binding. So stabilize the isolation and purification steps, while also efficiently removing chromosomal DNA and cell debris...particularly plasmids larger than 30 Kb. The novel "Lysis Viewer Reagent" is added to the reaction for a visual pH confirmation. Different colors indicate proper lysis and neutralization, making step protocol steps plain to see. The Fast DNA-spin Plasmid DNA Purification Kit can be used for both high-copy and low-copy plasmids so it is perfect for all your small-scale plasmid purification needs.



LITERATURE
User Manual - 50 samples
User Manual - 200 samples
 
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PCR Reagent Selection Chart

 

 

KIT CONTENTS
200 Column Kit
50 Column Kit
Part Numbers 17095, 17013
Resuspension Buffer 60 ml 15 ml
Lysis Buffer 60 ml 15 ml
Neutralization Buffer 80 ml 20 ml
Washing Buffer 50 ml 15 ml
Elution Buffer 30 ml 15 ml
Spin Columns 200 tubes 50 tubes
Collection Tubes 200 tubes 50 tubes
RNAase A, 10mg/ml 600 µl 150 µl
Lysis Viewer 300 µl 75 µl
Storage Room Temperature
Shelf Life 1 Year


ORDERING - Fast DNA-Spin Plasmid Purfication Kits, 17095 and 17013