| If you can't find the answer to your question, send an e-mail to us by clicking here and we will answer within 24 hours. | ||
| |
Questions |
Answers | |||
| Q1 |
Do you have any recommendations regarding the use of IgGZERO™ on primary cells regarding units per cell number? | A1 | We would suggest approximately 2000u/ 1 million cells. | |
| Q2 | Does IgGZERO™ also work below 37ºC? |
A2 | Yes, IgGZERO™ performs well in room temperature. If you would like to use it at room temperature, then add 15 min to the incubation time. . | |
| Q3 | Is the presence of anticoagulants, like EDTA, a problem for the enzyme activity? |
A3 | EDTA will not influence the activity of IgGZERO™. | |
| Q4 | Can I purify my IgG after deglycosylation with IgGZERO? |
A4 | Yes, because IgG molecules have an affinity for nickel-columns, it is best to use a Protein A column to bind the deglycosylated IgG and let the IgGZERO enzyme pass through. | |
| Q5 | Does IgGZERO leave the fucose intact when the GlcNAc is fucosylated? |
A5 | Yes. This makes it possible to analyze the degree of fucosylation. |