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FAQ - IgGZERO™ Endoglycosidase


Questions

Answers

Q1
Do you have any recommendations regarding the use of IgGZERO™ on primary cells regarding units per cell number?        A1

We would suggest approximately 2000u/ 1 million cells.

Q2

Does IgGZERO™ also work below 37ºC? 

  A2

Yes, IgGZERO™ performs well in room temperature. If you would like to use it at room temperature, then add 15 min to the incubation time.

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Q3

Is the presence of anticoagulants, like EDTA, a problem for the enzyme activity?      

  A3

EDTA will not influence the activity of IgGZERO™. 

Q4

Can I purify my IgG after deglycosylation with IgGZERO?      

  A4

Yes, because IgG molecules have an affinity for nickel-columns, it is best to use a Protein A column to bind the deglycosylated IgG and let the IgGZERO enzyme pass through.

Q5

Does IgGZERO leave the fucose intact when the GlcNAc is fucosylated?    

  A5

Yes. This makes it possible to analyze the degree of fucosylation.