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FAQ - YELLOW NIMT® Transfection Reagent


Questions

Answers

Q1
 What product can be used for transfection of DNA into suspension cells?   A1 YELLOW + NIMT®Booster when transfecting suspension cells with DNA.
Q2 What product can be used for transfection of DNA into adherent cells?   A2 Use YELLOW + NIMT®Booster when transfecting adherent cells with DNA.
Q3  Is it possible to separate transfected cells from untransfected cells by using a magnet?   A3 No. More than 95% of the cells take up YELLOW nanoparticles regardless of transfection efficiency. This means that some of the cells may not become transfected with DNA. If you then try to sort with a magnetic column, all cells that have taken up the particles will become sorted.
Q4 Will YELLOW work on my cell line?   A4 View the following table to find all cells that we know have been tested with YELLOW.
Q5 Is YELLOW toxic to cells?    A5  If you optimize the transfection according to protocol, YELLOW is not toxic. 
Q6 I would like to transfect cells with YELLOW + NIMT Booster ex vivo and transplant cells into an animal. Is it possible to track cells with MRI?   A6  Yes. All NIMT products are based on superparamagnetic nano particles and can therefore be visualized with MRI.
Q7  Is it possible to dilute YELLOW in serum free media, growth medium or buffers instead of water?     A7 No. To achieve optimal transfection efficiencies and to avoid toxic flocculations of the particles you should always use double distilled water when you dilute the particles and DNA prior to transfection. 
Q8  Is it possible to store and reuse diluted YELLOW particles?   A8  No. The transfection particles should always be diluted just prior to use.
Q9  When you add the transfection complexes to cells, the medium becomes diluted with water. How does that affect the cells?   A9 The cell proliferation may become affected to a small degree. If desired, the medium can be changed 6 hours after transfection but the efficiency of transfection may drop sligtly.
Q10   Are there any cell specific transfection protocols?   A10  No. To get the best transfection efficiencies it is better to do an optimization using your own cells, plate format and type of DNA. All instructions include an optimization protocol for adherent and suspension cell lines. We will gladly assist you to develop an optimization protocol, just click here to contact us.  
Q11  Is it possible to co-transfect DNA plasmids with YELLOW?   A11  Yes, just mix the DNAs into one solution and add to the particles and Booster according to the protocol.
Q12  Is it possible to co-transfect DNA and siRNA with NIMT®transfection reagents?      A12  We would recommend to first transfect your cells with DNA using YELLOW and NIMT®Booster. Incubate the cells for approximately 6 hours, change medium and then transfect cells with siRNA using PURPLE.