MBP-Trap Affinity Reagents

Capture MBP-tagged Proteins

Purify MBP-tagged proteins without contaminating peptides

The MBP-Trap is a single chain polypeptide antibody coupled to agarose beads for immunoprecipitation of MBP-tagged proteins. All Nano-Traps use a recombinant antibody fragment lacking a heavy chain. For this reason the MBP-Trap cannot dissociate from the agarose bead carrier, and therefore, offers the cleanest pulldown results. The MBP-Trap recognizes E.coli Maltose binding protein (MBP) which is often used as a fusion protein to increase solubility and expression level of a protein. Furthermore, MBP- tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect MBP fusion proteins. We guarantee you’ll see the fastest and most efficient performance! Typical results yield much lower background than other commercial or home-made affinity reagents. This make MBP-Trap perfect for labs who need crystal clean results for applications like mass spectrometry or other persnickety analyses



LITERATURE
Protocol
FAQs
 
RELATED PRODUCTS
Other Nano-Trap Reagents
Antibodies
Live Cell Imaging  Microscopes

 

 

Better than traditional monoclonal antibodiesImmunoprecipitation of MBP-tag pulldown

To the right is a pull-down experiment showing the MBP-Trap technology used during an immunoprecipitation of Maltose-binding protein from an E. Coli cell extract. Compared to Amylose, the MBP-Trap binds with a higher specificity to MBP. Therefore, the background is strongly reduced. The pulldown of MBP-fusion proteins is a fast and easy one-step IP that delivers reproducible data.

 

 

 

 

 

 

 

Nano-Traps

 

 

 

 

 

 

 

 

 

Camelidae Antibody

Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, dromedaries, llamas and alpacas) produce functional antibodies devoid of light chains, so called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW  of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity and affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.

 

 

 

 

SPECIFICATIONS

Specificity

E-coli

Configuration:

Specific Camelidae antibody linked to agarose bead

Part Numbers

MBTA010, MBTA020, MBTA100, MBTA200, MBTA400, MBTAK020, MBT250

Particle Size:

~90 µm  when coupled to an agarose beads
Storage Buffer: 20% EtOH

Storage and Stability:

store at 4°C; stable for one year. Do not freeze.


MBP Traps

ORDERING - MBP-Traps Coupled to Agarose

 

 

Binding Controls
Spin Columns for agarose beads