MK2-Trap Affinity Reagents

Novel Binding Protein for MK2 Studies

Analyze MK2 proteins in a tube

Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2 / MK2) belongs to the family of serine/threonine kinases. In response to cellular stress it is phosphorylated and activated by MAP kinase p38. Understanding how MK2 functions can hold the key to certain cell processes. One common avenue of research involves pulling down protein complexes from cell lysate and analyzing outside of the cellular milieu. The MK2-Trap is a high quality MK2-binding protein coupled to a monovalent matrix (agarose particles) for biochemical analysis of MK2 and interacting partners. With the MK2-Trap it is possible to yield a much cleaner result free from contaminants in under 30 minutes as compared with other pull-down techniques. MK2-Traps utilize super-high affinity Camelidae antibody fragments that may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nano-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. This affinity reagent is compatible with immunoprecipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.

 



LITERATURE
Protocols
FAQs
 
RELATED PRODUCTS
Other Nano-Trap Reagents
Antibodies
Live Cell Imaging  Microscopes

 

 

Nano-Traps

 

MK2 Western Blot

Pulldown of MK2 using the MK2-Trap

The proof is indisputable when using MK2-Trap – different forms of the MK2 protein can be isolated. To the left is a western blot of an immunoprecipitation (IP) of MK2 from a protein extract of HEK 293T cells. In this case, input (I), non-bound flow-through (FT) and bound (B) fractions were separated by SDS-PAGE followed by western blotting. MK2-Trap binds both the unphosphorylated MK2 and Phospho-MK2 (Thr222) forms of this protein. Specificity on Phospho-MK2 (Thr334) was not tested.

 

 

 

 

 

 

 

 

 

 

 

 

 

Camelidae Antibody

Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, dromedaries, llamas and alpacas) produce functional antibodies devoid of light chains, so called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW  of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity and affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.

 

 

 

 

SPECIFICATIONS

Configuration:

 

MK2-TrapA

Specific Camelidae antibody linked to agarose bead

Part Numbers
MTA010, MTA020, MTA100, MTA200, MTA400, MTAK020

MK2-Trap

Uncoupled and purified Camelidae antibody

Part Numbers
MT250

Particle Size:

 

MK2-Trap A

~90 µm when coupled to an agarose beads

MK2-Trap

No particle coupled

Storage Buffer:  

MK2-Trap A

20% EtOH

MK2-Trap

1x PBS; Preservative: 0.01% Sodium Azide

Storage and Stability:

 

MK2-Trap A

store at 4°C; stable for one year. Do not freeze.

MK2-Trap

store at 4°C; stable for one year. Do not freeze.



MK2 Traps

ORDERING - MK2-Traps Coupled to Agarose

 

 

Binding Controls
Spin Columns for agarose beads