M-Solution™
1-2 Antibiotics

Eliminates most cell culture mycoplasma

Nasty bugs are difficult to control

Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by penicillin or other antibiotics that target cell wall synthesis. Physically very small (< 1 micron), Mycoplasma are difficult to detect with a conventional microscope or neutralize with common antibiotics. As a result these robust bugs are often found in research laboratories as contaminants in cell culture. Reports indicate that 1/3 or more of all cell cultures are contaminated. Unfortunately, such contamination may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Moreover, severe Mycoplasma infections may destroy a cell line. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotech products.

Eradicates contamination 9 times out of 10

After using the eMyco PCR Detection Kit to positively identify cell culture contamination, eliminate the problem completely using the M-Solution™ 1-2 Antibiotics cocktail. M-Solution 1-2 Antibiotics (100x) are a combination of two different classes of antibiotics, which have been specifically designed to treat Mycoplasma infected cell cultures. This one-two punch has been demonstrated to knockout Mycoplasmas that can account for as much as 90% of all contamination issues. M-Solution 1 Antibiotic is based on the antibiotic Tiamutin and targets the 50S ribosomal sub-unit of many Microplasma strains – resulting in a break in the peptide chain immediately following initiation. M-Solution 2 Antibiotic is based on the antibiotic Minocycline, which is a broad spectrum tetracycline, and has a broader spectrum of susceptible species. Tetracycline antibiotics also are protein synthesis inhibitors, inhibiting the binding of aminoacyl-tRNA to the mRNA-ribosome complex. They do so mainly by binding to the 30S ribosomal subunit in the mRNA translation complex. Both antibiotics are bacteriostatic in effect and at higher concentrations can be lethal to Mycoplasma. These two antibiotics have been shown to be effective in eliminating the mycoplasma species frequently present in contaminated cell cultures. Furthermore, the mycoplasmas tend not to develop resistance to this cocktail – a common occurrence with other antibiotic treatment methods. The two antibiotic solutions are generally used sequentially, in combination over a period of 1 week, and the cycle is repeated 2-3 times, as required. The treatment is quite easy to perform and when used according to the following protocol, cytotoxic effects will be greatly ameliorated.