1) Cut out protein band and place in microfuge tube

2) Add 1 ml 30% ethanol or 30% acetone (30% acetic acid may be used but will result in acetylation of N-terminus).

3) Incubate for 30 minutes.

4) Repeat steps 2 and 3 until all stain removed from protein (typically 2 or 3 washes are required for complete stain removal)

5) Process as typical for mass spec analysis.