AcquaStain

Protein Gel Stain

Never Wash or Destain Again

Like Coomassie Blue only simpler

AcquaStain is a single-step Coomassie Blue protein gel dye.  This novel product doesn’t require pre- or post-treatment  of the gel.  Just run your protein gel, add the stain and watch your bands appear in several seconds. Say goodbye to destaining! AcquaStain contains no harmful chemicals or acids, so it's much safer than standard dyes. It can even be disposed of down the sink after staining. And because AcquaStain is a water-based product, no observable shrinkage or wrinkling of the gel occurs. Better yet, unlike methanol-based stains, proteins isolated from AcquaStain treated gels are 100% compatible with mass spectrometry analysis.

 

One-step in 10 minutes

Other “fast” gel staining technologies require expensive equipment or can poorly stain the protein bands.  And no other “fast” product is as fast as AcquaStain – completing the entire staining procedure in about 10 minutes (for typical protein amounts). Don’t worry though, it is impossible to over-saturate using this product, so you can incubate overnight with no harmful effects.  Only AcquaStain provides a reasonably-priced reagent without the need for an external apparatus while delivering a super sensitive signal – equivalent or better than the best Coomassie-based reagents on the market. Protein bands literally pop out of the gel because the gel background remains crystal clear. Acqua Stain is a brilliant blue color that's totally free from precipitate. Shaking before use is not necessary – it's like nothing you’ve tried before.



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LITERATURE
User Manual
MSDS
FAQs
 
RELATED PRODUCTS
Midori Green Safe Nucleic  Acid Staining Solution
Prestained Protein Ladders

FastGene LED Gel  Documentation System

 

 

 

Perfect for teaching labs

With the ability to run and stain protein gels during a typical one hour wet-lab class, this stain is right at home in the teaching lab. Want to make things even easier? Try our inexpensive, easy and portable gel documentation system, the FastGene GelPic LED Imaging System.

 

What customers are saying

“It works very well. It enabled some mass spectrometry experiments where we needed the sensitivity of silver staining but needed something compatible with mass spec. It was perfect for it.”
--Chad Rappleye, 2017
Ohio State University

 

Product Comparison

PRODUCT
NO PREWASH
NO DESTAIN
# OF STEPS
TOTAL TIME
SAFE DISPOSAL
CONC. LINEARITY
AcquaStain Protein Gel Stain
1
10 min
R2=0.98
Bio-Safe Coomassie Stain  
3
60-120 min
R2=0.71
EZBlue Gel Stain  
3
120-360 min
R2=0.91
Gelcode Safe Blue Protein Stain  
4
90-360+ min
R2=0.98
Simply Blue SafeStain  
3
360-420 min
R2=0.92

 

 

SPECIFICATIONS
Contents 1x 1000 ml bottle of liquid stain
Part Numbers AS001000
Toxicity Non-toxic (water-based)
Detection Limit Down to 10 ng of protein
Protocol Steps 1 step
Protocol Time

2 min stain time (down to 1 µg)

10 min stain time (down to 100 ng)

60 min stain time (down to 10 ng)

Suitable for Gel Drying Yes. Click for protocol.
Suitable for Mass Spec Yes. Click for protocol.
Reuseable Yes. 2-3 times
Storage 1 year at 4°C


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