DUALXtract Total Membrane Protein Extraction Kit

Efficient Isolation of Membrane Proteins

Get all of your membrane proteins from cells and tissue samples

The DUALxtract Total Membrane Protein Extraction Kit was developed to provide a simple and rapid procedure for isolating high quality membrane and cytoplasmic protein extracts without cross-contamination between the two fractions. The kit is based on stepwise differential protein solubilization. Treatment with different mild, non-denaturing detergent based buffers selectively separates membrane and membrane-associated proteins from the cytoplasmic fraction.

Cells are first permeabilized with the Cell Permeabilization Buffer to release the cytoplasmic protein fraction. The cell debris is then treated with the Membrane Protein Extraction Buffer which selectively solubilizes the majority of integral and membrane-associated proteins and isolates them as a separate fraction.

The kit is supplied with sufficient reagents for 50 extractions from 5 million cells or 20-40 mg of tissue per experiment.