e-Myco™ &
e-Myco plus

PCR Detection Kits

Detection of Mycoplasma in Cell Culture

Nasty bugs are difficult to control

Mycoplasma is a genus of bacteria which lack a cell wall. Without a cell wall, they are unaffected by penicillin or other antibiotics that target cell wall synthesis. Physically very small (< 1 micron), Mycoplasma are difficult to detect with a conventional microscope or neutralize with common antibiotics. As a result these robust bugs are often found in research laboratories as contaminants in cell culture. Reports indicate that 1/3 or more of all cell cultures are contaminated. Unfortunately, such contamination may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Moreover, severe Mycoplasma infections may destroy a cell line. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotech products.

PCR detection is the frontline for effective control

e-Myco™ and eMyco plus Mycoplasma PCR Detection Kit greatly simplify testing and detection of Mycoplasma contamination in cell cultures. With PCR testing, reliable results are obtained within a few hours, since the presence of contaminant Mycoplasmas can be easily and sensitively detected by verifying the amplified DNA fragments after gel electrophoresis. Both Mycoplasma PCR Detection Kits have been shown to be a highly sensitive, specific and rapid method for the detection of Mycoplasmas contamination in cell cultures. These kits can identify the five most common cell culture-infecting species of Mycoplasma all the way down to 20 cfu/ml. They also contain an internal control (primer set and template DNA) to identify false negatives caused by faulty PCR conditions and 8-methoxypsoralen (8-MOP) to prevent cross-contamination by PCR products from earlier experiments (8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent inter-strand crosslink after UV activation). Each tube of the e-Myco and eMyco plus Mycoplasma contains all the components for PCR (lyophilized) except for template: DNA Polymerase, dNTPs, PCR Buffer, primers set, 8-MOP, and internal control. So, you can just add your templates and perform the PCR reaction.

eMyco plus for the most demanding needs

e-Myco™ plus Mycoplasma PCR Detection Kit adds a measure of security when determining cell culture contamination issues. The breadth of detection is dramatically widened to include over 209 species amongst 8 genus, so that nary a mycoplasma can escape detection. In addition a sample control is included to test the veracity of the purified DNA, and is easily detected as a third unique band on an agarose gel.

* This kit is covered by patents owned by Abbott Molecular Inc. (US Pat. No. 5,851,767 and its foreign counterparts). For research use only.

ITEM #	SIZE	DESCRIPTION	PRICE	BUY
2523348	48 rxns	e-Myco Mycoplasma PCR Detection Kit	$125 $80*
25233	96 rxns	e-Myco Mycoplasma PCR Detection Kit	$225 $150*
2523448	48 rxns	e-Myco plus Mycoplasma PCR Detection Kit	$175 $115*
25234	96 rxns	e-Myco plus Mycoplasma PCR Detection Kit	$325 $220*

*Special pricing valid through June 30th, 2012