EasyClone
cDNA Library Construction Kit
Fast and Easy Synthesis of Highly Complex cDNAs
Directional cloning of cDNAs
The EasyClone cDNA Llibrary Construction Kit is designed to synthesize full-length-enriched
double stranded (ds) cDNA from total or polyA+ RNA, followed by subcloning into an appropriate library vector and amplification of the resulting cDNA library. The kit is based on a novel technology utilizing the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally library vectors for use with for use with yeast two-hybrid systems such as Dualsystems' DUALmembrane and DUALhybrid protein interaction screening kits.
No extensive know-how in library construction is needed - the kit supplies all reagents needed for library construction and the detailed manual will guide you through all steps of construction.
LITERATURE |
| User Manual |
RELATED PRODUCTS |
| EasyClone Normalized cDNA Library Construction Kit |
| EasyClone PCR Kit |
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Manufactured by  |
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KIT CONTENTS - Box 1 |
|
|---|---|
| 5X first strand buffer | 80 µl |
| DTT (20 mM) | 30 µl |
| 10X dNTP mix (10 mM each) | 25 µl |
| PlugOligo-1 adapter (15 µM) | 25 µl |
| CDS-1 adapter (10 µM) | 25 µl |
| PlugOligo-3M adapter (15 µM) | 25 µl |
| CDS-3M adapter (10 µM) | 25 µl |
| EasyClone reverse transcriptase | 20 µl |
| IP-solution | 130 µl |
| Control RNA template (0.5 µg/µl) | 15 µl |
| Sterile RNase free water | 1.8 ml |
KIT CONTENTS - Box 2 |
|
|---|---|
| 50X EasyClone polymerase mix | 50 µl |
| 10X EasyClone buffer | 300 µl |
| PCR Primer M1 (10 µM) | 100 µl |
| 50X dNTP mix (10 mM each) | 80 µl |
| Sterile RNase free water | 1.8 ml |
| Control amplified cDNA sample 1 | 25 µl |
| Control amplified cDNA sample 2 | 25 µl |
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| BUY
|
|---|---|---|---|---|
| P01010 | 20 Reactions |
EasyClone cDNA Library Construction Kit |
$1,350 |