FastGene

Direct PCR Kit

Go From Cells to PCR Product

PCR done the easy way

The Fastgene Direct PCR Kit is the optimal combination of our popular lysis reagent, DNArealeasy Advance, which releases DNA from cells and tissues in 15 minutes, and a second-generation KAPA2G Robust DNA Polymerase. This enzyme was created to achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the KAPA2G polymerase allows for consolidation of PCR cycling protocols and reaction conditions while maintaining or increasing amplicon success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.

 

Using the FastGene Direct PCR Kit is simple. Just mix cells with 30 μl of the included DNArealeasy Advance reagent, place in a thermal cycler or incubator and heat at 65°C for 5 minutes, followed by 96°C for 5 minutes before holding at 20°C for 5 minutes. After the lysis, a part or all of the lysate can be used directly with the included Kapa2G Robust HotStart ReadyMix and residual lysate can be stored at -20°C for future use.



LITERATURE
User Manual
 
RELATED PRODUCTS

DNArealeasy Adavnce Lysis  Reagent

BioReady rTaq Polymerase

Mini-Run Gel Electrophoresis  System

Midori Green Nucleic Acid  Staining Solution

Thermal Cyclers

 

 

DNAreleasy Advance results 1
DNAreleasy Advance results 2
Genomic DNA from scallops was isolated with DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield obtained. The same amplified DNA from scallops was used for sequencing resulting in high-quality reads.

 

DNAreleasy Advance results 3
DNAreleasy Advance results 4
Total DNA was isolated from different plant species using DNAreleasy Advance. Melting curves were determined by RT PCR:
Positive control, maize meal, wheat flour, sugar beet, white cabbage, negative control
Genomic DNA was isolated using DNAreleasy Advance and analyzed by qPCR:
(1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melangogaster, (6) horse meat
SPECIFICATIONS
Contents

DNAreleasy Advance Reagent

 

KAPA2G Robust HotStart Ready Mix

Part Numbers LS08, LS09
Hot Start Polymerase Yes, Ab-mediated
Amount of lysate directly used in PCR Up to 10%
SYBR Green Compatible Yes
Spectrophotometer Compatible No
Cell and Tissue Compatible Yes
Lysate Can Frozen for Use Later Yes, at -20°C
Storage 1 year at -20°C

 

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