FastGene RNA Premium Purification Kit

For Ultrapure & Highly Concentrated RNA

New level of RNA purity

The FastGene Premium RNA kit was developed for rapid, efficient, and clean purification of RNA from tissues and cells for challenging applications such as Next Gen sequencing (NGS). The kit’s silica-based technology dispenses with phenols in favor of superior DNase treatment to ensure the near-complete elimination of genomic DNA. It is the only RNA purification kit available that uses three separate column technologies. This results in extracted RNA of the highest concentration and purity – free from contaminating nucleic acids, proteins and other bio-molecules.

 

RNA of the highest quality

Historically, one of the most difficult aspects of RNA extraction has been ensuring that the final product is intact and of high quality. The quality of RNA is measured by determining a RIN (RNA integrity number). According to the manufacturer, RIN values above 8 are typically considered “high quality”. RNA purified with the FastGene RNA Premium Kit is consistently of higher quality RNA as compared to other kits (see figure below). The RNA is therefore in an ideal state for downstream applications, such as reverse transcription, qPCR and NGS.

 

RNA Quality

 

Fig. 1: RNA Quality determination using an Agilent Bioanalyzer. The FastGene Premium RNA Kit repeatedly delivers high quality RNA .



LITERATURE
User Manual
RELATED PRODUCTS

BioShake Plate Shakers

Magnetic Separation Stands

FastGene Plate Centrifuge
PCR Thermal Cyclers

PCR Reagent Selection Chart

 

 

Large yields

Obtaining a good yield from the RNA purification is essential. The FastGene RNA Premium Kit is optimized to deliver very large total yields. More RNA enables more analyses from each RNA prep.

 

RNA Yields

 

Highest concentration and near-perfect recovery

The FastGene Mini Elute column is an important component of the kit. Its unique design allows elution volumes as low as 10 µl.  Getting highly concentrated RNA stocks from a small amount of tissue or cellular material is a remarkable feat. Moreover, the recovery rate is very high (>95 %) even at very low elution volumes. The FastGene Premium RNA Kit provides a unique combination of benefits – high concentrations, high yields, high purity, and high recovery rates. See the results for yourself below:

 

RNA Concentration

 

SPECIFICATIONS
Part Number NGFG81006 NGFG81050 NGFG81250
Number Sample Preps 6 50 250
Lysis Buffer 4 ml 25 ml 125 ml
Wash Buffer 1 4 ml 35 ml 170 ml
Wash Buffer 2 2 ml 20 ml 2 x 50 ml
RNA Re-Binding Buffer 1 ml 8 ml 36 ml
Elution buffer (RNase free water) 1.5 ml 30 ml 200 ml
DNase I reconstitution solution 1.5 ml 1.5 ml 1.5 ml
10 x DNase I reaction buffer 50 µl 500 µl 2 x 1 ml
DNase I (lyophilized) 110 Kunitz units 110 Kunitz units 560 Kunitz units
FastGene RNA filter column 6 50 250
FastGene RNA binding column 6 50 250
FastGene RNA mini-elute column 6 50 250
1.5 ml collection tubes 12 100 500
2 ml collection tubes 18 150 750
FastGene mini-elute column (neutral color) Storage 2-8°C
FastGene RNA Premium Kit Storage Room Temp
Shelf Life 1 Year

 

FastGene RNA Premium Kit