FleX-IP Immuno-precipitation Kit
Detection of Protein Interactions by immunoreactivity
Complete and simple solution
The FleX-IP immunoprecipitation kit contains everything needed for highly efficient antigen immunoprecipitations using less than 10 µg of antibody. The specific antibody is first added to the sample to form an immune complex, which is then added to the Protein A/G agarose. The complex is washed to remove unbound material, and the bound immune complex is eluted for downstream applications like Western blotting.
The FleX-IP kit is optimally suited to confirm protein-protein interactions found in the DUALhybrid (yeast two-hybrid), DUALmembrane and DUALhunter screens by co-immunoprecipitation.
KIT CONTENTS |
|
|---|---|
| Protein A/G agarose | 800 µl (200 µl resin) |
| Control mouse IgG | 20 µl (1 mg/ml) |
| Lysis/Wash buffer | 50 ml |
| NaCl stock solution (5M) | 1 ml |
| SDS-PAGE buffer | 1 ml |
| Spin columns | 40 columns |
| Collection tubes | 40 tubes |
Ordering
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| BUY
|
|---|---|---|---|---|
| P01015 | 40 rxns |
FlexIP immunoprecipitation kit |
$320 |