GeneCellin DNA Transfection Reagent

From Delivery to Discovery

High efficiency and maximum viability

GeneCellin is a patented, polymer-based, in vitro transfection reagent which uses a completely novel type of reagent formulation. When compared with lipid-based transfection reagents, GeneCellin can achieve higher delivery efficiencies of plasmid DNA into cell lines and primary cells. Composed of nanoparticles, coined “nanoporters”, DNA is shuttled into suspension and adherent cells via the lysosome pathway. You’ll find that these simple-to-use nanoporters provide reproducible transgene expression levels with little, if any, cytotoxic effect. And because it’s a rapid, single tube, protocol compatible with serum, it is a pleasure to work with at your lab bench. See our growing list of tested cell types, published references and associated images. GeneCellin can be used for many common applications including: i) stable & transient transfections, ii) co-transfections, and even iii) reverse transfections. It’s not uncommon for us to hear researchers comment that they get twice the efficiency levels as their lipid-based reagents, and at a much lower cost.



 

 

LITERATURE
Quick-Start Guide
User Manual
List of Tested Cells
MSDS
FAQs
 
RELATED PRODUCTS
GeneCellin HTC DNA  Transfection Reagent
Transfection Tools
LumaScope Fluorescent Live  Cell Microscope

 

Transfect in serum

The presence of serum and antibiotics in the cell culture medium does not interfere with GeneCellin transfection efficiencies. Cells can be maintained in their regular culture medium during the transfection.

 

It’s smart to use less DNA

GeneCellin is so powerful that you can literally tune down your input DNA for each experiment. This saves costs and we’ve found that using less DNA reduces adverse cytotoxic effects triggered by over-expression, all while still maintaining high efficiency. See an example of HeLa cells transfected with sub-microgram levels of DNA. GeneCellin is a model of efficient delivery, in truest tradition of modern culture. We don’t want you to take our word for it though, so please contact us about receiving a free sample.

 

Customers love it

" Je souhaite vous transmettre toute ma satisfaction envers le réactif Genecellin, qui me permet de transfecter mes cellules avec un niveau d'efficacité jamais obtenu auparavant avec d'autres Réactifs.
Merci, c'est un merveilleux produit!" Université de Sherbrooke – Canada

“...very satisfied … this was the best transfection reagent she [sic] tested.“ Cancer Research Institute, Slovak Academy of Sciences – Slovakia

“...GeneCellin performed excellently in that it did not affect the expression …, was one of the least cytotoxic, and produced moderate to good transfection efficiency…” Monash University – Australia

 

SPECIFICATIONS
Part Numbers GC0500, GC1000, GC5000
Chemistry Type Polymer-based
Delivered Molecules DNA only
Cell Type Cell culture, in vitro
Cell Morphology Suspension and adherent
Amount per Transfection 0.5 µl to 1 µl (24-well plate)
Amount of Input DNA 0.25 µg to 1 µg (24-well plate)
Storage 1 year at 4°C


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