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FAQ - LPS Extraction Kit


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Q1
The instructions for LPS say that 5 ml of bacteria is the maximum volume that gives the optimum purity. What about if we increase the bacterial volume as well as the reagents. For instance, start with 30 ml of culture ( 6 x 5ml culture) and use the reagents 6x the recommended volume. Will the purity be still optimum?   A1 You can increase the starting volume. When doing this, you should increase the related reagents proportionally. We also recommend you to set the centrifuge over 10,000g in this case.