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FAQ - RedSafe™ Nucleic Acid Stating Solution


Questions

Answers

Q1
Is RedSafe suitable for UV illumination?   A1 Yes. RedSafe has two fluorescence excitation maxima when it is bound to nucleic acid. One is centered at 309 nm and another
is at 419 nm. In addition, it has one visible excitation of 514 nm (green).
Q2
Are the standard filters used for EtBr suitable for use with RedSafe?   A2 No. The red/orange filters for EB-stained gels should not be used with RedSafe stained gels. The filters for SYBR Green stained
gels (at 494nm and 521nm) should be used. In addition, yellow, green gelation or cellophane filters can be used.
Q3
Does RedSafe perform well with lower concentrations of DNA?   A3 Yes. However, smaller fragments of less than 300 bp
are not as bright as the larger ones.
Q4
Can we use RedSafe for RNA as well as DNA?   A4 Yes.
Q5
We usually add our stain directly to the gel. Is it okay to cast RedSafe directly in the gel?   A5 Yes. RedSafe can be cast directly in the gel, loaded along with the DNA sample or used as a post-stain.
Q6
Should we add RedSafe to the running buffer?   A6 RedSafe can be added to the running buffer (25µl per ml). In general, this is only necessary if you are having issues with sections of the gel washing out (lower or higher intensity).
Q7
How can RedSafe be disposed of after use?   A7 RedSafe does not create any toxic waste. Therefore, it
can be disposed according to laboratory regulations.
Q8
Can RedSafe be used as post-electrophoresis stain?   A8 Yes. Add 5µl of RedSafe to 100ml of buffer solution. You may need to adjust the concentration for optimal performance.
Q9
Will RedSafe work with low concentration of DNA ?   A9 RedSafe can work with low concentrations of DNA. However, smaller fragments of less than 300bp may not be as bright.
Q10
Will RedSafe work with DNA using polyacrylamide gels?   A10 Yes