i-D Maxime™ RT-PCR PreMix Kit

Freeze-dried Master Mix for One-Tube RT-PCR

Designed with diagnostic samples in mind

A one-tube RT-PCR kit requires a balance of performance, durability and repeatability. Developing the optimum enzyme and buffer configurations is critical to maintaining this balance. And when this kit is required to perform diagnostics analyses from a broad range of samples on different target genes, the most advanced technologies in enzyme mechanics and production techniques are mandatory. The i-D Maxime RT-PCR PreMix Kit is perfectly suited for diagnostic use. It utilizes a novel reverse transcription system with many of the benefits of both AMV and MMLV – similar to RevoScript. A one tube RT-PCR master mix, this product offers repeatable results for first-strand synthesis and amplification for a specific target sequence from RNA derived from viral, bacterial, plant or mammalian genome present in a variety of sample types. Each sealed 0.2 ml PCR tube

contains all components needed for gel-based analysis of your sample - just add template RNA and primers. The PCR phase utilizes hot start technology to reduce primer-dimer amplification, and results compare favorably with RT-PCR products from other name brand sources. Its simplicity is a hallmark of good SOP for diagnostic studies, reducing the chances of adding human error into sample analyses.

Reduce PCR carryover contamination

8-Methoxypsoralen (8-MOP) is a simple and inexpensive technology that can be used to eliminate contaminating DNA from earlier experiments. 8-MOP is known to intercalate into double stranded nucleic acids and form a covalent inter-strand crosslink after photo-activation by incident light at wavelength 320-400 nm. Samples can be treated after analysis to ensure that exposed amplicons won't serve as templates for future experiments.

Freeze-dried is not just for peas

Storage and ease of use are two often overlooked factors when working with reagents for molecular biology.  When all other things are equal, it's nice to know that your reagents can be just as effective on day 720 as on day 1.  And better yet, kits designed for minimum pipeting is just a smarter approach.  iNtRON has applied an elegant solution to these challenges.  A proprietary freeze-dried technique utilized in most of their molecular biology kits stabilizes the entire master mix at the bottom of 0.2 ml PCR tubes.  Then each strip of tubes is individually vacuum sealed to guarantee that no water infiltration can spoil the master mix over time.  Simply add sample (and/or primers) and your experiments are off to the races.   Eliminating preparation of master mixes and other components frees up time and reduces errors.  Whether it's PCR kits, diagnostic kits, or other enzyme kits, iNtRON products have incredibly long shelf lives for even the most finicky enzymes. We want you to be confident every time you purchase a PCR premix from us!

ITEM #	SIZE	DESCRIPTION	PRICE	BUY
25134	96 x 20 µl	i-D Maxime RT-PCR PreMix Kit *	$175 $95**

*not for human in-vitro diagnostic use

**Special pricing valid through June 30th, 2012