IgGZERO™
Endoglycosidase
Deglycosolates IgG molecules
Specifically remove oligosaccharides from the Fc region
IgGZERO™ is a very efficient endoglycosidase with high specificity for all four subclasses of IgG. The enzyme cleaves the chitobiose core of the glycan on IgG from various origin, such as human, rabbit, mouse, monkey, goat and sheep. This enzyme cuts the oligosaccharide chain at amino acid Asn-297. Click here for an illustration of precise cleavage site.
Scrub IgG from primary cell lines
IgGZERO™ endoglycosidase has a very high specificity for IgG molecules. The high efficiency of this endoglycosidase is very useful for quick removal of IgG molecules from Fc (gamma) receptors on primary cells. The enzyme is easy to use and allows further modification and conjugation of the IgG molecule after removal of the oligosaccharides.
Click here to see the effect of deglycosylating IgG Fc..
LITERATURE |
| Instruction Manual |
| Brochure |
| References |
| Immunology 2010 Poster |
| FAQs |
RELATED PRODUCTS |
| FabRICATOR® IgG Protease |
| FabRICATOR® F(ab’)2 Purification Kit |
| IgG Mass Spec Kit |
|
Manufactured by |
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SPECIFICATIONS |
|
|---|---|
| Total Protocol Length | 30 minutes |
| Total Protocol Steps | 2 steps |
| Typical Yield | 1 unit cleaves >95% IgG 1ug Glycan |
| Specificity | Single site at hinge region | Storage | -20°C |
| Shelf Life | 1 year @-20°C, 1 month @4°C |
| Microbe Free | Yes, lot tested |
ITEM #
| SIZE
| DESCRIPTION
| PRICE
BUY
| |
|---|---|---|---|---|
| A0-IZ1-010 | 1,000U | IgGZERO™, Cleaves 1mg IgG |
$275 |
|
| A0-IZ1-050 | 5,000U | IgGZERO™, Cleaves 5mg IgG |
$1,095 |
|
| A0-IZ1-250 | 25,000U | IgGZERO™, Cleaves 25mg IgG |
$4,495 |
|
| A0-IZ1-999 | Custom | IgGZERO™, Bulk quantity |
Price on request |
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