Firefly Luciferase Assay Kit

Accurate and Sensitive Detection in Cultured Cells

Light it up for less

Luciferase is one of the most commonly used light-producing proteins for molecular biology assays. The Firefly Luciferase Assay Kit (from the firefly Photinus pyralis) is an accurate, sensitive and easy method for studying gene reporter regulation and function. Designed for use with transformed cells in culture, the protocol takes about 15 minutes with two easy steps: lyse cells and add substrate. The 100 µl reaction volume is perfect for detecting results in a 96-well plate format.

 

The Firefly Luciferase that is included in this kit as a control has a molecular weight of 62 kDa, is active as a monomer, and does not require subsequent processing for its activity. The enzyme catalyzes the oxidation of reduced luciferin in the presence of ATP-Mg2+ and oxygen to generate CO2, AMP, PPi, oxyluciferin, and a flash of light that is proportional to the quantity of luciferase in the reaction mixture. Get your glow on!

 

The Luciferase Assay Substrate includes coenzyme A, ATP and luciferin. Including coenzyme A in the reaction enhances the sensitivity of the assay and provides a sustained light reaction (half-life > 5 minutes). This eliminates the need for automated luminometer injection of substrate and allows analysis by photographic film or scintillation counting. G



LITERATURE
User Manual
 
RELATED PRODUCTS
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Luciferase Assay

 

SPECIFICATIONS
Size 100 assays
Includes

10ml Luciferase Assay Substrate

4ml Cell Lysis Buffer (5x)

10 µg Luciferase (Control)

Shelf Life 1 Year
Storage -20°C, protect from light


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