Mammalian Expression Vectors

Strong CMV Promoter for High-level, Constitutive Expression

Sfi I sites for convenient, directional cloning of full-length cDNAs

Sfi I is a rare restriction enzyme which recognizes the sequence GGCCNNNNNGGCC. The variable core sequence allows the design of multiple Sfi I sites with incompatible overhangs within the same vector. Dualsystems vectors feature two Sfi I sites with the sequences GGCCATTACGGCC and GGCCGCCTCGGCC, allowing directional cloning of cDNAs. Sfi I sequences are extremely rare in eukaryotic genomes and therefore, the majority of eukaryotic cDNAs can subcloned full-length using Sfi I.

pHA-MEX / pFL-MEX

Mammalian expression vectors featuring a strong CMV promoter, an N-terminal HA or ECS epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pHA-MEX and pFL-MEX are suited for both transient overexpression and long-term, stable expression. 

pMEX-HA / pMEX-FL

Mammalian expression vectors featuring a strong CMV promoter, a C-terminal HA or ECS* epitope tag for detection of expressed proteins and a NeoR cassette for stable integration of the expression vector into the genome of the host cell. pMEX-HA and pMEX-FL are suited for both transient overexpression and long-term, stable expression.

ITEM #	SIZE	DESCRIPTION	PRICE	BUY	MAP	SEQUENCE
P03401	5 µg	pHA-MEX	$520
P03402	5 µg	pMEX-HA	$520