Mdm4/HdmX-Trap Affinity Reagents

Novel Binding Protein for p53 Studies

Analyze Mdm4/HdmX proteins in a tube

The protein called “double minute 4” (Mdm4/HdmX/ MdmX) plays a role in cell cylce control and apoptosis by inhibiting p53 and p73. A tool for isolating this protein from cell lysates can help your lab get a better picture of how Mdm4 and associated proteins work within these essential cell functions. Mdm4/HdmX-Trap is a 1-step pull-down kit which provides better results than other techniques. Yields are higher, purity is cleaner, and contaminant free pull-downs are achieved in under 30 minutes. The Mdm4 HdmX-Trap is a high quality Mdm4 HdmX-binding protein i.e. Nanobody) coupled to a monovalent matrix (agarose particles) for biochemical analysis of Mdm4/HdmX and interacting partners. Note that binding of Mdm4/HdmX-Trap interferes with binding of p53 to Mdm4 HdmX.

 

Mdm4/HdmX-Traps utilize super-high affinity Camelidae antibody fragments that may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nano-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. This reagent allows Mdm4/HdmX to be the perfect candidate for immunoprecipitations, Co-IP, and mass spectroscopy.



LITERATURE
Protocols
FAQs
 
RELATED PRODUCTS
Other Nano-Trap Reagents
Antibodies
Live Cell Imaging  Microscopes

 

 

 

Nano-Traps

 

Mdm4 Western Blot

Pulldown of Mdm4/HdmX using the Mdm4/HdmX-Trap

To the right is an immunoprecipitation (IP) of Mdm4/ HdmX from a protein extract derived from HEK 293T cells. Fractions were run out on an SDS-PAGE gel and transferred to a blot for immuno-identification with a classical ant-MDMX antibody. Results of this western blot analysis clearly show the enrichment of the Mdm4 protein around 80 kDa (this protein has been observed to have aberrant migration on gels not indicative of it 55kDa predicted mass). Input (I), non-bound (FT) and bound (B) fractions show a high and specific binding capacity for this Nano-Trap.

 

 

 

 

 

 

 

 

 

 

 

 

Camelidae Antibody

Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, dromedaries, llamas, and alpacas) produce functional antibodies devoid of light chains, so called "heavy chain" antibodies. Heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW of 13 kDa). Called “Nanobodies”, these proteins are characterized by high specificity, affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.

 

 

 

 

SPECIFICATIONS

Configuration:

 

Mdm4/ HdmX-TrapA

Specific Camelidae antibody linked to agarose bead

Mdm4/ HdmX-Trap

Uncoupled and purified Camelidae antibody

Particle Size:

 

Mdm4/ HdmX-Trap A

~90 µm when coupled to an agarose beads

Part Numbers
HTA010, HTA020, HTA100, HTA200, HTA400, HTAK020

Mdm4/ HdmX-Trap

No particle coupled

Part Numbers
HT250
Storage Buffer:  

Mdm4/ HdmX-Trap A

20% EtOH

Mdm4/ HdmX-Trap

1x PBS; Preservative: 0.01% Sodium Azide

Storage and Stability:

 

Mdm4/ HdmX-Trap A

store at 4°C; stable for one year. Do not freeze.

Mdm4/ HdmX-Trap

store at 4°C; stable for one year. Do not freeze.



Mdm4/HdmX Traps

ORDERING - Mdm4/HdmX-Traps Coupled to Agarose

 

 

Binding Controls
Spin Columns for agarose beads