Midori Green

Nucleic Acid Staining Solution

Replace your toxic EtBr!

Thoroughly tested for your safety

Midori Green Nucleic Acid Staining Solution is a new and safe alternative to traditional ethidium bromide(EtBr) stain for detecting dsDNA, ssDNA and RNA in agarose gels. Nearly identical to EtBr in performance and use, Midori Green is much less harmful to living organisms. As compared with EtBr, which is known as a strong mutagen, Midori Green causes many fewer mutations in the Ames test. When compared with spontaneous mutations caused by water in the Ames test, Midori green rate was only slightly beyond the standard deviation range. In addition (and in contrast to EtBr), Midori Green scores negative in the mouse bone marrow micronucleus test and in the chromosome aberration test which indicates it’s considered non-carcinogenic. And for those who prefer to be safe, rather than sorry, Midori Green has been tested for latex/nitrile glove penetration, in which both materials show negative results, even after 6 hrs of exposure. Most importantly, Midori Green is not considered hazardous waste, and can be disposed of according to standard laboratory procedures.

 



LITERATURE
Instructions - Midori Green  Advance
Instructions - Midori Green  Direct
MSDS - Midori Green Advance
MSDS - Midori Green Direct
Safety Report- Midori Green  Advance
Safety Report - Midori Green  Direct
White Paper - RNA Staining
FAQs
RELATED PRODUCTS

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Fastgene Agarose Gel Band  Cutter

 

Itís a happy family of nucleic acid stains

The core Midori Green molecule has been formulated into two different stains optimized for your lab’s needs. First, there is Midori Green Advance - which offers an excellent signal-to-noise ratio and very high sensitivity for even the shortest nucleic fragments. Second is Midori Green Direct which is designed to just mix with your sample and load onto a stain-free gel. MG Direct has the loading dye included and shows performance similar to Midori Green Advanced.

 

Safer for you and better for your subcloning

By using safe DNA dyes and safe LED illuminators you can improve your subcloning transformation efficiencies by THREE-FOLD. In this example, a plasmid vector was double digested with suitable restriction enzymes to create two sticky-ended DNA fragments: the lacZ gene (3,536 bp) and the backbone of the vector (4,318 bp). Equal amounts of digested DNA were electrophoresed on 1% agarose gels. The gels were stained with either ethidium bromide or Midori Green Direct gel stain according to the corresponding manuals, and then viewed using either a UV transilluminator or the FastGene Blue/Green LED Illuminator, respectively. The two DNA fragments were excised from the gels and purified using a silica membrane based purification kit. The lacZ gene and the vector backbone were religated using T4 DNA ligase transformed into DH5a cells and plated onto selection plates. The total number of blue and white colonies was counted to evaluate cloning efficiency. Each experiment was conducted in triplicate, and the average cloning efficiency was determined. Midori Green Direct resulted in dramatic increase of positive transformants.

CFUs are higher with Midori Green Gel Comparison

Midori Green Can Boost Your Cloning Results!

 

Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short wave-length light is well known to cause thymidine dimers and damage the DNA. The extent of this damage is not always appreciated. High energy light wreaks havoc on a DNA fragment in mere seconds. As can be seen below, after only a 15 sec exposure of DNA in a standard agarose gel, cloning efficiency starts to drop. And after a 30 sec exposure your cloning experiment is all but dead! In contrast, the cloning efficiency of protocols that use Blue LEDs seem completely immune to any deleterious effects. If your lab isn't able to break itself of its ethidium bromide habit, using a Blue/Green LED illuminator (and imaging systems) have no significant impact on cloning efficiency or DNA integrity.

 

UV exposure is bad for cloning

UV Transilluminators Kill Cloning Experiments

 

Just as sensitive as EtBr

Both dyes can stain single- and double-stranded nucleic acids with sensitivity and protocols similar to that of EtBr. This makes it a no-brainer as a drop-in replacement. Best yet, not only can you use your existing UV transilluminator and filter sets, Midori Green also works well when matched with Blue LED Gel Illuminators. These harmless light tables are the perfect complement for these stains, as you can work shield-less and goggle-less when excising bands or imaging migration. And because blue light does not cross-link DNA, there is never a worry about damaging DNA during cloning and photographing sessions.

     1 kb DNA ladder stained with Midori Green
     and EtBr

100bp DNA ladder stained with Midori Green and EtBr
   
SPECIFICATIONS
  Midori Green Advance Midori Green Direct
Part Numbers MG04

MG06

Contents 1ml tube (20,000x)

1ml tube

(10x loading dye)

Add to Gel Yes No
Add to Sample No Yes
UV Transilluminator Compatible Yes Yes
Blue LED Illuminator Compatible Yes (side illumination recommended) Yes
Excitation

270 nm

290 nm
490 nm

290 nm
500 nm

Emission 530nm 530nm
Toxicity

Non-toxic

Non-carcinogenic

Non-mutagenic

Storage 1 Year in the dark at 4°C


ORDERING - Midori Green DNA Stains