NEPA21
Electro-Kinetic Transfection System

Versatile Electroporator for In Vivo, In Vitro and Much More

High efficiency and high viability without special buffers

The most popular transfection procedures all require specialized chemicals which are costly – running as much as $8, $12 or even $16 or more per sample. These additives can have unwanted effects on the cell and at the minimum create another parameter which must be considered. Though poking holes in cell membranes has been around for many years, no protocols exist for high efficiency transfections without the use of such reagents. That is, not until the NEPA 21. Nepagene has created THE most sophisticated apparatus for delivering DNA and RNA to cells. And all that is required are the electrodes. With a combination of low voltage short-and-long pulses, with reversing polarities, a huge variety of cells, tissues and even organisms can be transfected with amazingly high

efficiencies. This patented pulsing technology has been shown to be both superior and far more flexible than other techniques. And because everything works at low voltages, cytotoxic effects are nearly eliminated. Read more about the secrets of NEPA 21’s technological breakthrough.

In vitro transfection of suspension, adherent and primary cells

The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives. Simply add cells and pulse – then save money and time. For adherent cells, use a special cell-culture-plate electrode for 2-dimensional electro-kinetic transfer of RNA and DNA. Defying conventional wisdom, the patented electric pulsing not only pricks channels in the plasma membrane, but also the nuclear envelope, allowing DNA to be transported into the cell nucleus for efficacious transfection results with primary cells.

In vivo transfection in mice and rats

The NEPA21 works with a plethora of electrodes that puts Dr. Frankenstein to shame. These specialized tools can be used to gently pulse organs and tissues in live animals. The electric fields carry DNA and RNA molecules directly into the cells that make up these tissues. These tools allow previously difficult, or impossible, transfection experiments to be executed, opening new avenues of scientific inquiry. Transfections have been positively performed on muscle, skin, liver, kidney, testis, ovary, brain, retina, cornea and others in mice and rats. Click on the following tissue types to see the specific electrodes and protocols designed for each: brain, retina, muscle, skin and testis. And if you need to study tissue outside of the organism, the NEPA 21 is amenable to ex vivo transfer of nucleic acids to a variety of tissue types including brain.

In utero, ex utero and in ovo transfections for developmental studies

Nepagene has developed tools and techniques for transfecting embryonic tissues, or even whole embryos, in mice, rats and chickens. Whether it is specific regions in a mouse embryonic brain, or chicken cortex or gastro regions we can provide a protocol and an electrode set for that. The flexibility is almost limitless as these electrodes have been adopted for use with a variety of other animals and plants including honey bee, xenopus and even plant seeds. Give us a call to discuss the options in more detail.

Click here to see a complete list of applications with protocols.

Click here to see available electrodes for In Vivo, In Utero, In Ovo and Ex Vivo applications.

Ordering

ITEM #	SIZE	DESCRIPTION	PRICE	BUY
NEPA21	1	NEPA 21 Electro-kinetic Transfection System	Inquire
C200	1	Foot switch for NEPA21	Inquire
CU500	1	Cuvette adaptor for suspension Cells	Inquire
CUY650P5	1	Electro-kinetic tweezers w/5mm platinum disk electrodes for mouse embryos	Inquire
CUY611P7-2	2	Electro-kinetic platinum chopstick electrodes, 7mm bend, 2mm tip lenghth, 2/package	Inquire
CUY701P7E	1	Electro-kinetic 7mm x 7mm platinum square electrode on petridish w/1mm height frame	Inquire
CU710S	1	Oscilloscope and Attenuator for NEPA21	Inquire
CUY900-13-3-5	1	Electro-kinetic adaptor for adherent cells - 24-well culture plate	Inquire
		In Vivo, In Utero, In Ovo and Ex Vivo Electrodes Click here to view current offering	Inquire