NEPA21
Electro-Kinetic Transfection System

Versatile Electroporator for In Vivo, In Vitro and Much More

High efficiency and high viability without special buffers

The most popular transfection procedures all require specialized chemicals which are costly running as much as $8, $12 or even $16 or more per sample. These additives can have unwanted effects on the cell and at the minimum create another parameter which must be considered. Though poking holes in cell membranes has been around for many years, no protocols exist for high efficiency transfections without the use of such reagents. That is, not until the NEPA 21. Nepagene has created THE most sophisticated apparatus for delivering DNA and RNA to cells. And all that is required are the electrodes. With a combination of low voltage short-and-long pulses, with reversing polarities, a huge variety of cells, tissues and even organisms can be transfected with amazingly high efficiencies.This patented pulsing technology has been shown to be both superior and far more flexible than other techniques. Relying upon unique non-capacitor driven pulsing, the NEPA21 works at low voltages to deliver perfect square waves with no degradation. As compared with cheaper models, results with the NEPA21 are often far better when it matters most. And because everything works at low voltages, cytotoxic effects are nearly eliminated. Read more about the secrets behind NEPA 21s proprietary pulsing technology.



 

For 2017 we are offering a $3,000 discount on NEPA21 systems! Contact us for quote.

 

 

LITERATURE
Brochure
References
Available Electrodes
FAQs
 
RELATED PRODUCTS

ELEPO21 In Vitro High  Voltage Electroporator

Electroporation  Cuvettes
LumaScope Fluorescent Live  Cell Microscopes
Bambanker Cell Freezing  Media

 

 

In vitro transfection of suspension, adherent and primary cells

The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives. Simply add cells and pulse then save money and time. For adherent cells, use a special cell-culture-plate electrode for 2-dimensional electro-kinetic transfer of RNA and DNA. Defying conventional wisdom, the patented electric pulsing not only pricks channels in the plasma membrane, but also the nuclear envelope, allowing DNA to be transported into the cell nucleus for efficacious transfection results with primary cells, stem and ES cells, as well as iPS cells.

 

In vivo transfection in mice and rats

The NEPA21 works with a plethora of electrodes that puts Dr. Frankenstein to shame. These specialized tools can be used to gently pulse organs and tissues in live animals. The electric fields carry DNA and RNA molecules directly into the cells that make up these tissues. These tools allow previously difficult, or impossible, transfection experiments to be executed, opening new avenues of scientific inquiry. Transfections have been positively performed on muscle, skin, liver, kidney, testis, ovary, brain, retina, cornea and others in mice and rats. Click on the following tissue types to see the specific electrodes and protocols designed for each: brain, retina, muscle, skin and testis. And if you need to study tissue outside of the organism, the NEPA 21 is amenable to ex vivo transfer of nucleic acids to a variety of tissue types including brain.

 

In utero, ex utero and in ovo transfections for developmental studies

Nepagene has developed tools and techniques for transfecting embryonic tissues, or even whole embryos, in mice, rats and chickens. Whether it is specific regions in a mouse embryonic brain (click for a movie), or chicken cortex or gastro regions we can provide a protocol and an electrode set for that. The flexibility is almost limitless as these electrodes have been adopted for use with a variety of other animals and plants including honey bee, xenopus and even plant seeds. Give us a call to discuss the options in more detail.

 

Click to see a complete list of data for suspension cells, adherent cells, or In Vivo/In Utero/In Ovo/Ex Vivo electroporation applications (supplier website).

 

Click here to see available electrodes for In Vivo, In Utero, In Ovo and Ex Vivo applications.

 

 

SPECIFICATIONS
# pulsing phases Up to 4
Types of pulses Poring and Transfer
# of poring pulses 0-9
# of transfer pulses 0-99
Decaying pulses Yes
Reverse polarity Yes
Impedance readout Yes
Total energy readout Yes, in Joules
Power Requirements AC100-240V 50/60Hz
Size 346(W) x 330(D) x113(H) mm
Weight 7.5 kg
Warranty 2 Year


Ordering

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
NEPA21
1
NEPA 21 Electro-kinetic Transfection System (Nepa21 Super Electroporator)
Inquire
C115CB-2
1
Female plug & Cover banana plug cable, red & black, 2M, for NEPA21/ECFG21
Inquire
C117
1
Hook & male plug cable, red & black, for NEPA21/ECFG21
Inquire
C118
1
2-Female to 1-Male plug cable, red & black
Inquire
C200
1
Foot switch for NEPA21
Inquire

 

Popular Electrodes (please contact us if you would like us to recommend an electrode for your electroporations):

 

In Vitro Electroporation (suspension cells)

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CU500
1
Cuvette electrode adaptor for suspension cells
Inquire
CU600
1
Clear stand that holds up to 20 cuvettes upright, numbered slots
Inquire
12358-346 50
Pkg of 50 individually wrapped (2mm) electroporation cuvettes
Click to go to product page

 

Adherent Cell Electroporation (not trypsinized)

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY900-13-3-5
1
NEPA21 electrode for adherent cells - 24-well culture plate
Inquire

 

In Vivo Zygote Electroporation

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY520P5
1
Petridish Parallel Platinum Plate Electrode
L8 x W5 x H3mm, 5mm gap
Inquire
CUY5001P1-1.5
1
20mm length platinum plate electrodes on petridish, 1mm gap, 1.5mm height (5-20ul volume)
Inquire
CUY501P1-1.5
1
20mm length platinum plate electrodes on glass slide, 1mm gap, 1.5mm height (5-20ul volume)
Inquire

 

In Utero Embryo Electroporation

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY650P3
1
Tweezer w/3mm platinum disk electrodes for mouse embryos in utero
Inquire
CUY650P5
1
Tweezer w/5mm platinum disk electrodes for mouse embryos in utero
Inquire
CUY650P5X4
1
Tweezers w/5x4mmφ platinum oval electrodes for mouse embryos in utero
Inquire
CUY650P7
1
Tweezer w/7mm platinum disk electrodes for mouse embryos in utero
Inquire

 

Skin and Muscle Electroporation

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY568-4
1
3 x 5mm needle electrode (3mm spacing) with center hole for syringe (syringe is not included and should be 29G x 13mm)
$1,995
CUY692-21
1
21 needle electrode, 14mm tip length, 0.5mmφ for muscle
Inquire
CUY692-21-1
1
21 needle electrode, 1mm tip length, 0.5mmφ for skin
$1,995
CUY663P3X6
1
Tweezers w/3 needle fork & 3mm x 6mm platinum rectangular electrodes for skin and tumors
Inquire
CUY663P8X10
1
Tweezers w/3 needle fork & 8mm x 10mm platinum rectangular electrodes for skin and tumors
Inquire

 

Tissue Explant Electroporation

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY520P5
1
Petridish Parallel Platinum Plate Electrode
L8 x W5 x H3mm, 5mm gap
Inquire
CUY520P15
1
Petridish Parallel Platinum Plate Electrode
L10 x W15 x H5mm, 15mm gap
Inquire
CUY700P1E
1
1mm platinum disk electrode in petridish
Inquire
CUY700P1L
1
1mm platinum disk electrode on tip of stick
Inquire
CUY700P2E
1
2mm platinum disk electrode in petridish
Inquire
CUY700P2L
1
2mm platinum disk electrode on tip of stick
Inquire
CUY700P3E
1
3mm platinum disk electrode in petridish
Inquire
CUY700P3L
1
3mm platinum disk electrode on tip of stick
Inquire
CUY700P4E
1
4mm platinum disk electrode in petridish
Inquire
CUY700P4L
1
4mm platinum disk electrode on tip of stick
Inquire
CUY700P5E
1
5mm platinum disk electrode in petridish
Inquire
CUY700P5L
1
5mm platinum disk electrode on tip of stick
Inquire
CUY700P7E
1
7mm platinum disk electrode in petridish
Inquire
CUY700P7L
1
7mm platinum disk electrode on tip of stick
Inquire
CUY700P10E
1
10mm platinum disk electrode in petridish
Inquire
CUY700P10L
1
10mm platinum disk electrode on tip of stick
Inquire
CUY700P20E
1
20mm platinum disk electrode in petridish
Inquire
CUY700P20L
1
20mm platinum disk electrode on tip of stick
Inquire
CUY701P2E
1
2mm x 2mm platinum square electrode in petridish
Inquire
CUY701P2L
1
2mm x 2mm platinum square electrode on tip of stick
Inquire
CUY701P5E
1
5mm x 5mm platinum square electrode in petridish
Inquire
CUY701P5L
1
5mm x 5mm platinum square electrode on tip of stick
Inquire
CUY701P7E
1
7mm x 7mm platinum square electrode in petridish
Inquire
CUY701P7L
1
7mm x 7mm platinum square electrode on tip of stick
Inquire
CUY701P20E
1
20mm x 20mm platinum square electrode in petridish
Inquire
CUY701P20L
1
20mm x 20mm platinum square electrode on tip of stick
Inquire

 

Other Electrodes

ITEM #
SIZE
DESCRIPTION
PRICE
BUY
PDF
CUY200S
1
Electrode set for brain including CUY202, CUY207, CUY208, & CUY580
Inquire
CUY580
1
Electrode holder for CUY200S, CUY550-10, CUY611 and CUY613 series
Inquire
CUY560-3-0.3
1
Parallel Fixed 2 Needle Straight Tip Electrode 10mm-Tip, 0.3mm diameter, 3mm gap
Inquire
CUY560-5-0.5
1
Parallel Fixed 2 Needle Straight Tip Electrode 10mm-Tip, 0.5mm diameter, 5mm gap
Inquire
CUY567-0.5
1

Parallel w/ Variable Gap 2 Needle Electrode 10mm-Tip, 0.5mm diameter, 1mm to 20mm gap

Inquire
CUY610P4-1
1
Platinum parallel fixed needle electrodes, 4mm gap, 1mm tip length, for chick embryos
Inquire
CUY611P2-1
2
Platinum chopstick electrodes, 2 mm bend, 1mm tip lenghth, 2/package
Inquire
CUY611P3-1
2
Platinum chopstick electrodes, 3mm bend, 1mm tip lenghth, 2/package
Inquire
CUY611P7-2
2
Platinum chopstick electrodes, 7mm bend, 2mm tip lenghth, 2/package
Inquire
CUY611P7-4
2
Platinum chopstick electrodes, 7mm bend, 4mm tip lenghth, 2/package
Inquire
CUY613P1
2
Z-shape chopstick platinum electrodes, 1mm tip length, 0.3mmφ, 2/pkg
Inquire
CUY613P3
2
Z-shape chopstick platinum electrodes, 3mm tip length, 0.3mmφ, 2/pkg
Inquire
CUY614T
5
Replacement tungsten needle electrodes, 200μmφ, 5/pkg
Inquire
CUY615
1
Electrode holder for CUY614 and CUY614S
Inquire
CUY650P0.5
1
Tweezer w/0.5mm platinum disk electrodes
Inquire
CUY650P0.5-3
1
Tweezer w/0.5mmφ & 3mmφ platinum disk electrodes
Inquire
CUY650P1
1
Tweezer w/1mm platinum disk electrodes
Inquire
CUY650P1-5
1
Tweezer w/1mm & 5mm platinum disk electrodes
Inquire
CUY650P3
1
Tweezer w/3mm platinum disk electrodes for mouse embryos in utero
Inquire
CUY650P5
1
Tweezer w/5mm platinum disk electrodes for mouse embryos in utero
Inquire
CUY650P5X4
1
Tweezers w/5x4mmφ platinum oval electrodes for mouse embryos in utero
Inquire
CUY650P7
1
Tweezer w/7mm platinum disk electrodes for mouse embryos in utero
Inquire
CUY650P10
1
Tweezer w/3mm platinum disk electrodes
Inquire
CUY651P
1
Tweezer w/platinum U-shape platinum electrodes, outer: 6mmφ inner: 3mmφ - For spinal cord of mouse embyo
Inquire
CUY661-3X7
1
Tweezers w/7mm length tungsten
needle & 3mm x 7mm ellipse stainless steel electrodes
Inquire
CUY661N
5
Tungsten replacement needle for CUY661-3X7, 5/pkg
Inquire
CUY663P3X6
1
Tweezers w/3 needle fork & 3mm x 6mm platinum rectangular electrodes for skin and tumors
Inquire
CUY663P8X10
1
Tweezers w/3 needle fork & 8mm x 10mm platinum rectangular electrodes for skin and tumors
Inquire
CUY671P1
1
Platinum parallel fixed needle electrodes, 0.5mmφ, 1mm gap, 45 degree
Inquire
CUY675P5
1
Tweezers w/horseshoe electrode, 5mmφ for mouse retina
Inquire
 
request quote for custom electrodes
Inquire