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Specialized Enzymes for Working with IgGs

Dear Reader,

 

Hello from Bulldog Bio!  You're a first time receiver of our newsletter - The Barker.  We understand the value of your time, and so we only wish to present unique opportunities for improving the quality of your research experience.  Thanks for taking time to read more about a novel endoglycosidase for highly specific and efficient removal of sugar groups from IgG molecules.

 

Sincerely,

 

Bulldog Bio

Best in Show - IgGZERO™ Endoglycosidase

 

IgGZERO is the first known bacterial enzyme with a unique specificity for removing common carbohydrates from native IgG. The enzyme cleaves the chitobiose core of the glycan on IgG from various origins, such as human, rabbit, mouse, monkey, goat and sheep. And after its job is done, an engineered histidine-tag allows IgGZERO to be easily extracted from the freshly modified IgGs. Antibodies without this oligosaccharide are now ready for further modification and conjugation. For example, this allows quick removal of IgG molecules from Fc(gamma) receptors on primary cells. If this enzyme sounds of interest for your research, then click here to request a sample.

 

Advantages

  • Hydrolyzes conserved sugar group from IgGs
  • Efficient activity with native or denatured IgGs
  • Very simple and fast protocol
  • Compatible with broad range of IgG types

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    The Fc portion of IgG contains a conserved carbohydrate attached to the asparagine 297 residue in the CH2 domain of each heavy chain. The presence of this carbohydrate is crucial for proper antibody structure and interactions with cellular IgG Fc gamma receptors (FcgR) and the complement system. IgGZERO™, a recombinant form of EndoS, has a specific endoglycosidase activity for native IgG by hydrolyzing the conserved glycans attached to the asp 297 residue on the IgG heavy chains. The activities of other known endoglycosidases require, or are enhanced by, denaturation of the glycoprotein substrate. Click here to read publications related to the characterization and function of EndoS from Streptococcus pyogenes.

    K-9 Logic

     

    Every quarter we give away 30 scientist bobble heads to subscribers to The Barker who can answer a riddle or logic problem. If you know the answer to this month's challenge, click here to submit your answer and grab a chance to win a free Pillars of Science bobble head.


    Once again, our post-doctoral protagonist Dr. Green faces an interesting challenge to her daily routine. This time the lab's precision electronic balance has gone on the fritz. And she needs to quickly determine which of her knockout mice is exhibiting an obesity-related gene. In this case, only 1 of the 27 mice in the colony is actually exhibiting the phenotype of increased body weight. By chance, or perhaps because of her phenomenal experimental technique, the remaining 26 mice are nearly the same weight. Dr. Green was able to rummage through a storage room and locate a classic 2-pan balance (i.e. with opposing pans in which two objects' weights can be directly compared). What are the fewest rounds of measurements required to exactly determine the obese mouse without relying upon luck?


    For example - if there were 8 mice you might say 3 rounds (first, 4 mice against 4 mice, then take the heavier group and divide into groups of 2 to be measured, and finally take the remaining 2 and compare them directly to determine the heaviest mouse).