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A New Way to Transfect Primary Cells

Dear Reader,

 

Hello from Bulldog Bio! You're a first time receiver of our newsletter - The Barker. We understand the value of your time, and so we only wish to present opportunities relevant to your research. If you prefer not to receive The Barker, please unsubscribe at the bottom of this email. Thanks for taking time to read more about a new technology for delivering DNA and RNA into difficult-to-transfect primary cells.

Best in Show -
NIMT® Transfection Reagents

 

Primary cell lines behave finicky for various transfection methods and products.  One key to maximizing transfection effectiveness is to deliver nucleic acids without perturbing the cells' metabolic state.  NIMT Transfection Reagents do just this - by achieving high transfection rates without causing major changes to cell viability.  The secret is the use of tiny, lipid-coated, iron oxide particles to both carry and deliver nucleic acids.  This technology is widely applicable.  YELLOW NIMT has been used to successfully boost transfection rates and reduce cytotoxicity for transient DNA transfections and PURPLE NIMT does the same for siRNA delivery, plus speeds-up knockdown effects. Click here to request a sample of YELLOW or PURPLE Transfection Reagent.

 

Advantages

  • Low toxicity to many cell types
  • Compatible with primary cells
  • Highly efficient transfection rates
  • Simple and fast to use
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    Win a free bobble head in K9 Logic below!

     

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    If you are ordering any of our Transfection Tools products for the first time, we will include a case of 1000 Aloetouch ICE Nitrile Lab Gloves for free (a $95 value).  Just reference the following promotional code in your order: BTB002.  Your hands will thank you!


     

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    Finding the most effective transfection method can be an important consideration for researchers who work with primary cell cultures.  NIMT Transfection Reagents work by a novel, nanopartcle-mediated, uptake through the endocytosis pathway of cells.  Within minutes these nanoparticles are absorbed by most cells at an incredible 95% efficiency.  And because relatively few particles are needed to deliver sufficient quantities of nucleic acids, the cells themselves tend to stay healthy throughout the transfection protocol.  Read some of our recent Applications Notes on how YELLOW NIMT and PURPLE NIMT can be used to transfect traditionally difficult cell lines.

     

    K-9 Logic

     

    Every month we give away 30 scientist bobble heads to subscribers to The Barker that can answer a riddle or logic problem. If you know the answer to this month’s challenge, click here to submit your answer and have a chance to win a free Gregor Mendel bobble head.

     

    You may recall that in a well-known laboratory there are three post-doctoral researchers, Dr. Green, Dr. Purple and Dr. Orange. Interestingly, and not without its own set of problems, Dr. Green always lies, Dr. Purple alternatively lies and tells the truth, and Dr. Orange always tells the truth.

     

    One day the lab had a visitor who knew the researchers by their first names only (in no particular order – Emma, Ling and Rama) and also a spouse of one of the post docs, Ulli. As the visitor had forgotten the surnames of the three post-docs and to which Ulli was married, she decided to ask each post-doc two questions: i) their last name and ii) to whom was Ulli married.
    Emma responded “I am not Dr. Green, and Ulli is married to Dr. Purple.”
    Ling replied “I am not Dr. Purple and Ulli is married to Dr. Orange.”
    And Rama retorted “I am not Dr. Orange and Ulli is married to Dr. Green.”
    So to whom is Ulli married?