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A New Class of Reverse Transcriptase

Hello from Bulldog Bio!

 

We're on the cusp of adding two new partners from Europe and Asia respectively.  But this hasn't stopped us from continuing to expand our broad range of tools for molecular and cellular biology.  This month we're highlighting a new type of reverse transcriptase not previously available in the US.  Read about RevoScript, below, and you'll understand why this new class of RTase might be just what your lab needs.

Best in Show - RevoScript™ RT Premix Kit

 

Retroviruses have always been a hot research topic and interesting products for bench research sporadically arise from these endeavors.  Such is the case for RevoScript, a reverse transcriptase isolated from the well characterized avian reticuloendotheliosis retrovirus.  This new class of RTase combines the best characteristics of the two most popular enzymes - MMLV and AMV.  What makes RevoScript so revolutionary is that it produces high quality, high yield cDNAs at higher temperatures.  This combination minimizes secondary structure effects of RNA and maximizes the quality of the cDNAs.  It is typical to get cDNAs ranging up to 9 kb with great yields from even low abundance mRNAs.  Revoscript is supplied in a master mix with either random hexamers or oligo (dT) primers.  And better yet, this concoction is lyophilized so you never need to worry about storage or stability issues.  All you need to do is ask for a sample, add RNA, and join the revolution!

 

Advantages

  • Higher temperature stability beyond 60°C
  • Higher yields of cDNA products
  • Very sensitive sub-picogram cDNA synthesis
  • Super stable and convenient lyophilized mix

  • Gradient PCR for $3495

     

    Throw Me a Bone

     

    We provide a total satisfaction guarantee for all reagent purchases.  This means if you're not 100% satisfied, just contact us and we'll reimburse you the price of the product.

     

    And any time you purchase one of our thermal cyclers, well give you 125x 8-Strip PCR Tubes with attached Caps, 2K units of BioReady rTaq and 48 tubes of RevoScript RT Premix. Refer to BTB1104.


    Now new scientist bobble heads can be won below!

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    A Dog's Life - "real lab bench experience"

     

    Storage and ease of use are two often overlooked factors when working with reagents for molecular biology.  When all other things are equal, it's nice to know that costly kits can be just as effective on day 360 as on day 1.  And better yet, kits designed for the minimum of pipeting is just a smarter approach.  iNtRON has applied an elegant solution to these challenges.  Their proprietary lypholization technique utilized in most of their molecular biology kits stabilizes the entire master mix.  Simply add sample and your experiments are off to the races.   Eliminating preparation of master mixes and other components frees up time and reduces errors.  Whether it's PCR kits, diagnostic kits, or other enzyme kits, iNtRON products have incredibly long shelf lives (over 1 year) for even the most finicky enzymes.  Take for example RevoScript from this month's spotlighted product - typically reverse transcriptases are one of the most fragile enzymes, however Revoscript's all-in-one kits are bullet proof and guaranteed for at least one year in the freezer.

     

    K-9 Logic

     

    Every calendar quarter we give away 30 scientist bobble heads to subscribers to The Barker who correctly answer a riddle or logic problem. If you know the answer to the Q2 challenge, click here for a chance to win a free Pillars of Science bobble head

     

    This time we join Dr. Orange in the midst of a conversation with the undergraduate students in her fly genetics lab. She is trying to hammer home some simple concepts regarding fly genetics, and decides that a little mathematics problem is the best way to get their neurons firing. Dr. Orange says "Class, here is a lab culture vial containing seventeen flies with one of four non-lethal eye phenotypes: white eyes, orange eyes, red eyes, and eyeless" as she shows them the vial. "Whoever can count the number of flies with wild-type red eyes will earn extra credit today." She proceeds to allow each student thirty seconds to try and count the wild-type flies. Soon after they start, the students complain it is just too hard to count the peppy flies. She then proffers up the final bits of information necessary to solve the problem, "Oh, and by the way, flies with red eyes are the most abundant. Neither of the four phenotypes have the same number in the vial. There are at least two of each color. And if I randomly pick enough flies, and only just enough flies, from the culture to ensure that I have at least two of any one eye type and one of any second phenotype, I must pick 11 flies." Can you answer Dr. Orange's question about the number of wild-type red eyes correctly and earn a bobble head?