Non-Normalized cDNA Libraries
Improve the Quality of Yeast Two-hybrid Screens
You may also view our normalized cDNA libraries
Unique improvements
These high quality cDNA's are constructed using a brand new technology. Dualsystems utilizes the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. Differences in length of the 5’ adaptor primer ensure transcription of all three reading frames.
Packaged for success
Libraries are supplied as 200 µg or 60 µg purified plasmid DNA that is ready for screening. 200 µg is sufficient for seven library scale screens and 60 µg is sufficient for two library scale screens. cDNAs are cloned into pGAD-HA or pGAD-DS (N-terminal GAL4-fusion) for DUALhybrid libaries or pPR3-N (N-terminal NubG-fusion) or pPR3-C (C-terminal NubG-fusion) for DUALmembrane & DUALhunter libaries.
LITERATURE |
| Brochure |
RELATED PRODUCTS |
| Normalized cDNA Libraries |
| DUALhybrid Kits |
| DUALmembrane Kits |
| DUALhunter Kits |
|
Manufactured by  |
|
SPECIFICATIONS |
|
|---|---|
| Clones | > 1x10E6 |
| Inserts | > 85% plasmids containing inserts |
| Average insert size | at least 1.2 kb |
| Number of screens | 7 (200 µg library) 2 (60µg library) |
Yeast two-hybrid (DUALhybrid) Non-Normalized cDNA libraries
ORDER
|
|---|
Mouse
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02101 | 200 µg |
Mouse 11d total embryo |
$1,500 |
 |
| P12101 | 60 µg |
Mouse 11d total embryo |
$750 |
|
| P02107 | 200 µg | Mouse adult heart |
$1,500 |
|
| P12107 | 60 µg | Mouse adult heart |
$750 |
|
| P02112 | 200 µg | Mouse adult ovary |
$1,500 |
|
| P12112 | 60 µg | Mouse adult ovary |
$750 |
|
| P02117 | 200 µg |
Mouse cortical neurons |
$1,500 |
|
| P12117 | 60 µg |
Mouse cortical neurons |
$750 |
Human
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02102 | 200 µg | Human colon |
$1,500 |
|
| P12102 | 60 µg | Human colon |
$750 |
|
| P02106 | 200 µg |
Human hepatocellular carcinoma cells |
$1,500 |
|
| P12106 | 60 µg |
Human hepatocellular carcinoma cells |
$750 |
|
| P02103 | 200 µg | HeLa cell line |
$1,500 |
|
| P12103 | 60 µg | HeLa cell line |
$750 |
|
| P02104 | 200 µg |
LNCaP human prostate cancer cells |
$1,500 |
|
| P12104 | 60 µg |
LNCaP human prostate cancer cells |
$750 |
|
| P02114 | 200 µg |
MCF-7S1 (mammary gland adenocarcinoma) |
$1,500 |
|
| P12114 | 60 µg |
MCF-7S1 (mammary gland adenocarcinoma) |
$750 |
|
| P02116 | 200 µg |
HUVEC (umbilical vein endothelial cells) |
$1,500 |
|
| P12116 | 60 µg |
HUVEC (umbilical vein endothelial cells) |
$750 |
Model Organisms
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02105 | 200 µg |
C. elegans whole adult |
$1,500 |
|
| P12105 | 60 µg |
C. elegans whole adult |
$750 |
|
| P02108 | 200 µg |
Saccharomyces cerevisiae |
$1,500 |
|
| P12108 | 60 µg |
Saccharomyces cerevisiae |
$750 |
|
| P02109 | 200 µg |
Arabidopsis thaliana |
$1,500 |
|
| P12109 | 60 µg |
Arabidopsis thaliana |
$750 |
|
| P02110 | 200 µg | Plasmodium falciparum |
$1,500 |
|
| P12110 | 60 µg | Plasmodium falciparum |
$750 |
|
| P02111 | 200 µg |
Chlamydomonas reinhardtii |
$1,500 |
|
| P12111 | 60 µg |
Chlamydomonas reinhardtii |
$750 |
|
| P02113 | 200 µg | Arabidopsis thaliana floral bud |
$1,500 |
|
| P12113 | 60 µg |
Arabidopsis thaliana floral bud |
$750 |
|
| P02115 | 200 µg |
Zebrafish PGC 9-12h |
$1,500 |
|
| P12115 | 60 µg |
Zebrafish PGC 9-12h |
$750 |
Yeast two-hybrid (DUALmembrane & DUALhunter) Non-Normalized cDNA libraries
ORDER
|
|---|
Mouse
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02201 | 200 µg | Mouse adult whole brain / NubG-x |
$2,500 |
|
| P12201 | 60 µg | Mouse adult whole brain / NubG-x |
$1,100 |
|
| P02206 | 200 µg |
Mouse adult heart / x-NubG |
$2,500 |
|
| P12206 | 60 µg | Mouse adult heart / x-NubG |
$1,100 |
|
| P02207 | 200 µg |
Mouse adult heart / NubG-x |
$2,500 |
|
| P12207 | 60 µg |
Mouse adult heart / NubG-x |
$1,100 |
|
| P02231 | 200 µg |
Mouse adult kidney / NubG-x |
$2,500 |
|
| P12231 | 60 µg |
Mouse adult kidney / NubG-x |
$1,100 |
|
| P02224 | 200 µg | Mouse adult spleen / x-NubG |
$2,500 |
|
| P12224 | 60 µg | Mouse adult spleen / x-NubG |
$1,100 |
|
| P02234 | 200 µg |
Mouse whole embryo, 11 days / NubG-x |
$2,500 |
|
| P12234 | 60 µg |
Mouse whole embryo, 11 days / NubG-x |
$1,100 |
|
| P02246 | 200 µg | Mouse adult testis / NubG-x |
$2,500 |
|
| P12246 | 60 µg | Mouse adult testis / NubG-x |
$1,100 |
Rat
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02233 | 200 µg | Rat neonatal cardiomyoctes / NubG-x |
$2,500 |
|
| P12233 | 60 µg | Rat neonatal cardiomyoctes / NubG-x |
$1,100 |
Human
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02205 | 200 µg |
Jurkat T cells, unstimulated / NubG-x |
$2,500 |
|
| P12205 | 60 µg |
Jurkat T cells, unstimulated / NubG-x |
$1,100 |
|
| P02212 | 200 µg |
HeLa cell line / NubG-x |
$2,500 |
|
| P12212 | 60 µg |
HeLa cell line / NubG-x |
$1,100 |
|
| P02228 | 200 µg |
LNCaP cell line / NubG-x |
$2,500 |
|
| P12228 | 60 µg |
LNCaP cell line / NubG-x |
$1,100 |
|
| P02232 | 200 µg |
Mammary epithelial cells / NubG-x |
$2,500 |
|
| P12232 | 60 µg |
Mammary epithelial cells / NubG-x |
$1,100 |
|
| P02240 | 200 µg |
Hep-G2 (hepatocellular carcinoma) / x-NubG |
$2,500 |
|
| P12240 | 60 µg |
Hep-G2 (hepatocellular carcinoma) / x-NubG |
$1,100 |
|
| P02221 | 200 µg | Human adult brain / NubG-x |
$2,500 |
|
| P12221 | 60 µg | Human adult brain / NubG-x |
$1,100 |
|
| P02227 | 200 µg |
Human adult brain / x-NubG |
$2,500 |
|
| P12227 | 60 µg |
Human adult brain / x-NubG |
$1,100 |
|
| P02213 | 200 µg | Human embryonal brain / NubG-x |
$2,500 |
|
| P12213 | 60 µg | Human embryonal brain / NubG-x |
$1,100 |
|
| P02215 | 200 µg |
Human adult colon / NubG-x |
$2,500 |
|
| P12215 | 60 µg |
Human adult colon / NubG-x |
$1,100 |
|
| P02220 | 200 µg | Human adult liver / NubG-x |
$2,500 |
|
| P12220 | 60 µg | Human adult liver / NubG-x |
$1,100 |
|
| P02226 | 200 µg | Human adult kidney / NubG-x |
$2,500 |
|
| P12226 | 60 µg | Human adult kidney / NubG-x |
$1,100 |
|
| P02208 | 200 µg | Human adult kidney / x-NubG |
$2,500 |
|
| P12208 | 60 µg | Human adult kidney / x-NubG |
$1,100 |
|
| P02230 | 200 µg |
Human adult lung / NubG-x |
$2,500 |
|
| P12230 | 60 µg |
Human adult lung / NubG-x |
$1,100 |
|
| P02245 | 200 µg |
Adult human small intestine / NubG-x |
$2,500 |
|
| P12245 | 60 µg |
Adult human small intestine / NubG-x |
$1,100 |
Model Organisms
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02229 | 200 µg | Drosophila melanogaster / NubG-x |
$2,500 |
|
| P12229 | 60 µg | Drosophila melanogaster / NubG-x |
$1,100 |
|
| P02218 | 200 µg | Caenorhabditis elegans adult / NubG-x |
$2,500 |
|
| P12218 | 60 µg | Caenorhabditis elegans adult / NubG-x |
$1,100 |
|
| P02222 | 200 µg | Caenorhabditis elegans eggs / NubG-x |
$2,500 |
|
| P12222 | 60 µg | Caenorhabditis elegans eggs / NubG-x |
$1,100 |
|
| P02237 | 200 µg | Saccharomyces cerevisiae / NubG-x |
$2,500 |
|
| P12237 | 60 µg | Saccharomyces cerevisiae / NubG-x |
$1,100 |
|
| P02238 | 200 µg | Zebrafish brain / x-NubG |
$2,500 |
|
| P12238 | 60 µg | Zebrafish brain / x-NubG |
$1,100 |
|
| P02244 | 200 µg | Zebrafish brain / NubG-x |
$2,500 |
|
| P12244 | 60 µg | Zebrafish brain / NubG-x |
$1,100 |
|
| P02241 | 200 µg | Ustilago maydis / NubG-x |
$2,500 |
|
| P12241 | 60 µg | Ustilago maydis / NubG-x |
$1,100 |
|
| P02243 | 200 µg |
Dictyostelium discoideum / NubG-x |
$2,500 |
|
| P12243 | 60 µg |
Dictyostelium discoideum / NubG-x |
$1,100 |
Plant
ITEM #
| SIZE
| DESCRIPTION
| PRICE
| DATA SHEET
|
|---|---|---|---|---|
| P02210 | 200 µg |
Arabidopsis thaliana / NubG-x |
$2,500 |
|
| P12210 | 60 µg |
Arabidopsis thaliana / NubG-x |
$1,100 |
|
| P02239 | 200 µg | Arabidopsis thaliana / x-NubG |
$2,500 |
|
| P02239 | 60 µg | Arabidopsis thaliana / x-NubG |
$1,100 |
|
| P02235 | 200 µg | Medicago nodules / x-NubG |
$2,500 |
|
| P12235 | 60 µg | Medicago nodules / x-NubG |
$1,100 |
|
| P02236 | 200 µg |
Medicago nodules / NubG-x |
$2,500 |
|
| P12236 | 60 µg |
Medicago nodules / NubG-x |
$1,100 |