p53-Trap Affinity Reagents

Novel Binding Proteins for p53 Studies

Analyze p53 in a tube

The tumor suppressor protein p53 plays a major role in cell cycle control, apoptosis and senescence. Understanding the details of how p53 works within these important cell processes is a focus for many laboratories. One key technique for studying complex cell processes is a pull-down experiment. The p53-Trap is a high quality p53-binding protein coupled to a monovalent matrix (agarose particles) for biochemical analysis of p53 and interacting partners. With the p53-Trap, it is possible to yield ultra-clean result free from contaminants in under 30 minutes. p53-Traps utilize super-high affinity Camelidae antibody fragments that may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nano-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. This reagent allows p53 to be perfect candidate for immunoprecipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.

 



LITERATURE
Protocol p53-N-Term-Trap
Protocol p53-C-Term-Trap
FAQs
 
RELATED PRODUCTS
Other Nano-Trap Reagents
Antibodies
Live Cell Imaging  Microscopes

 

 

Nano-Traps

 

p53 Western Blotp53 C-term Trap Western Blot

Pulldown of p53 using the p53-Traps

To the right is a western blot demonstrating the effectiveness of p53 from whole cell extracts. In this case, an immunoprecipitation (IP) of p53 from a protein extract of HEK 293T cells was performed with p53-Trap. Western analysis of the input (I), non-bound flowthrough (FT) and bound (B) fractions were separated by SDS-PAGE followed by western blotting.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Camelidae Antibody

Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, dromedaries, llamas and alpacas) produce functional antibodies devoid of light chains, so called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW  of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity and affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.

 

 

 

 

SPECIFICATIONS

Configuration:

 

p53-TrapA

Specific Camelidae antibody linked to agarose bead

Part Numbers
PTA010, PTA020, PTA100, PTA200, PTA400, PTAK020, PTA210, PTA220, PTA2100, PTA2200, PTA2400, PTAK220

p53-Trap

Uncoupled and purified Camelidae antibody

Part Numbers
PTA250, PTA2250

Particle Size:

 

p53-Trap A

~90 µm when coupled to an agarose beads

p53-Trap

No particle coupled

Storage Buffer:  

p53-Trap A

20% EtOH

p53-Trap

1x PBS; Preservative: 0.01% Sodium Azide

Storage and Stability:

 

p53-Trap A

store at 4°C; stable for one year. Do not freeze.

p53-Trap

store at 4°C; stable for one year. Do not freeze.



P53 N-Term-Traps (Note that binding of p53-Trap N-term interferes with binding of Mdm4/HdmX to p53)

ORDERING - P53 N-Traps Coupled to Agarose

 

 

P53 C-Term-Traps

ORDERING - P53 C-Term-Traps Coupled to Agarose

 

 

Binding Controls
Spin Columns for agarose beads