PRO-PREP Protein Extraction Solution

Extracts your proteins without degradation

Proteins love the freezer

Extracting proteins from mixtures of cells or tissues is a long standing art in the world of life science research. Every lab seems to have a favorite technique for particular cell lines or tissue types. Wouldn't it be nice to have a single, go-to, protocol to replace these multitude of techniques? And wouldn't it be better if the protocol were simple, short and extraordinarily effective? This is the case for Pro-Prep Protein Extraction Solution. A unique cocktail of detergents, buffers and protein inhibitors is specially designed and extensively tested for easy extraction or mammalian, insect or any cells without walls ... and all without degradation. The trick is performing the extraction at -20°C in a buffer that solubilizes, denatures and monomerizes proteins. The protocol takes less than 45 minutes from start to finish. Four steps later you can stably store your extracted proteins in the freezer for up to 6 months. The Pro-Prep buffer is formulated for accurate protein measurements with a Bradford assay as their is no interfering absorbance. The denatured monomers are ideal for SDS-PAGE followed by western blot analysis.

 



LITERATURE
User Manual
MSDS
 
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SPECIFICATIONS
Part Numbers 17081, 17081.1
Contents 100ml bottle
Added protease inhibitors

PMSF (phenylmethylsulfonyl fluoride)

EDTA (17087 only)
Pepstatin A

Leupeptin

Aprotinin

Target proteins

Effective for extracting cytoplasmic, membrane and nuclear proteins

Denaturing Yes, SDS-free
Tissue or cell type Animal derived - cells without walls
Storage 1 year at -20°C (avoid frequent freeze-thaws)


ORDERING - PRO-PREP Protein Extraction Solution