QuickLyse Vector pEAS-1a

Rapid Autolysis of Bacterial Strains

Recombinant proteins purified directly from the supernatant

The novel QuickLyse vector pEAS-1a provides rapid, temperature-controlled lysis of bacteria expressing your recombinant protein of interest. Just clone your gene into pEAS-1a, transform into an appropriate expression host and induce protein expression, followed by a temperature shift to induce autolysis. There is no need for lysosyme treatment or sonication.  After lysis, the recombinant protein is immediately accessible for downstream purification and analysis.

The QuickLyse system uses the autolytic genes derived from the SRRz cluster of phage Lambda (Kloos et al., 1994). The SRRz genes are under the control of the heat inducible cI857/pR promoter from phage Lambda and the strong rrnB terminator. Shifting cells from 28°C to 38°C induces expression of the SRRz genes, resulting in quick and efficient autolysis of bacterial cells.The system is compatible with common E. coli expression strains, such as BL21 or  XL1-Blue.