Basic information to run and maintain your NEPA21 system
The following will help any lab using a NEPA21 system:
- How to program the NEPA21
- Why do you need to record electrical parameters?
- How to clean platinum electrodes
- Lost the original user manual? Request a replacement
The following will help ensure the safe shipment of the NEPA21 system from your lab:
- How to repack the NEPA21
- Don’t have the original box? Request NEPA21 packing materials
Specific details for common protocols using the NEPA21 system
The NEPA21 has been used in dozens of published applications. There are a few important details to manage for this system, so we encourage you to thoroughly review the topics on this page as they relate to your lab’s experiments. If you have any questions or concerns, first contact us before starting a new type of experiment — we’re happy to help!
Check out these common protocols for cell lines, primary cells, and cell clusters:
- How to electroporate cells in suspension using electroporation cuvettes
- How to electroporate cells in adherence using adherent cell electrodes
Check out these common protocols for creating transgenic animals using CRISPR/Cas9 Reagents:
- How to genetically edit zygotes using platinum electrode chambers
- How to genetically edit zygotes within oviducts of living mammal using platinum tweezer electrodes
Key Publications for NEPA21 Applications and Cell Types
The NEPA21 has been used with hundreds of cell lines, primary cell types and much more. Following are some common (and not-so-common) types of cells that have already been transfected with the NEPA21. Check out “real lab” data from labs throughout the world. [CV = cell viability, TE = transfection efficiency]
|
Cell Type
|
Field Results
|
Field Data
|
Key Publication
|
|---|---|---|---|
|
Caco-2
|
CV = 85% – 95%
TE= 80%
|
Download | Link |
|
CHO
|
CV = 74% – 98%
TE= 80% – 99%
|
Download | |
|
Embryonic Stem
|
CV = 55% – 80%
TE= 55% – 100%
|
Download | |
|
iPSCs
|
CV = 80%
TE= 86%
|
Download | Link |
|
Jurkat
|
CV = 80% – 100%
TE= 50% – 68%
|
Download | |
|
Organoids
|
CV = 80% – 100%
TE= 50% – 68%
|
Download | Link |
|
Primary Fibroblasts
|
CV = 65% – 95%
TE= 50% – 95%
|
Download | |
|
Primary Immune
|
CV = 48% – 93%
TE= 41% – 90%
|
Download | |
|
Primary Neuronal
|
CV = 60% – 91%
TE= 55% – 80%
|
Download | |
|
RAW
|
CV = 70% – 100%
TE= 56% – 80%
|
Download | |
|
SH-SY5Y
|
CV = 60% – 79%
TE= 60% – 90%
|
Download | |
|
THP-1
|
CV = 56% – 85%
TE= 63% – 85%
|
Download |
Can’t find the cell type that you’re interested in? Just contact us—we’d be happy to perform an up-to-the-minute search for you.