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Q1 – Can CleanNGS be used for RNA clean-up?
Yes. CleanNGS is formulated for use with ssDNA, RNA, and PCR products.
Q2 – What is the recommended ratio for RNA clean-up?
The volume ratio of 1.8X beads-to-sample is recommended for RNA clean-up.
Q3 – What are the smallest fragments that can be purified with CleanNGS beads?
The lower limit for selection with these beads is somewhere between 50 bases and 100 bases.
Q4 – How should I prepare gDNA for use with CleanNGS beads?
When working with gDNA, shear to <3,000bp fragments before using with CleanNGS beads.
Q5 – What can I do to help increase the yield of extraction?
To increase the yield of a DNA/RNA extraction with a CleanNA kit, you can preheat the elution buffer to 70°C before elution.
Q6 – What is the binding capacity of CleanNGS Beads?
Typically about 40% of input DNA/RNA can be bound and released by CleanNGS beads. The absolute upper limit of binding for 1 uL of beads can be above 5 ug of nucleic acid.
Q7 – Are CleanNGS Beads stable at room temperature?
Absolutely. In fact we ship the product at room temperature with no degradation to performance. Once open, the bottle should be stored at 4°C.
Q8 – Can CleanNGS Beads withstand freeze-thaw cycles?
No. This is why the beads should never be stored in the freezer.