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Q1 – What is the binding capacity of the Nano-Trap (GFP-Trap/ RFP-Trap)?
That depends on the beads. Agarose beads usually binds at least 5 µg GFP per 10 µl slurry, the magnetic particles around 0.25 – 0.5 µg per 10 µl slurry.
Q2 – Will I be able to elute bound proteins from the GFP-Trap?
For quantitative elution you can either boil the sample for 10 minutes at 95° C in SDS sample buffer or incubate for 0.5 to 2 minutes in 0.2M glycine pH 2.5, followed by neutralization with 1/10 vol 1M Tris base.
Q3 – Is it possible to elute bound proteins from the GFP-Trap in their native state, e.g. for gel shift experiments or functional assays?
You may try to elute with free GFP. However, please be aware that this method will not quantitatively elute your fusion protein of interest.
Q4 – How can I avoid unspecific protein interactions binding to the GFP-Trap?
The critical step is to dilute the concentration of the detergent in the incubation buffer. We recommend a final concentration of 0.1% of the detergent (e.g. NP-40 or TX-100) and a final volume of at least 0.4 ml. In addition we recommend to test various salt concentrations in the wash buffer (e.g. 150 mM – 500 mM NaCl) to remove unspecifically bound hydrophilic proteins.
Q5 – Will the eluted GFP binding protein cross-react with a secondary Ig specific antibody that is used to detect an antigen-specific first antibody?
Since the binding protein used in the GFP-Trap does not have any significant homology with goat, mouse, rat or human antibodies, unspecific reactions with a secondary Ig specific antibody should not occur.
Q6 – Is there a difference in binding when I use N-terminal vs. C-terminal GFP fusions?
The GFP-Trap has a slightly higher affinity for C-terminal GFP-fusions. You can compensate this by an elongated incubation time ( 1 – 2 h instead of 15 – 30 min)
Q7 – Can I purify GFP labeled fusion proteins directly from tissue samples, i.e. in a denaturing buffer?
In principle the GFP-Trap is very stable even under harsh buffer conditions (e.g. RIPA buffer containing 0.1% SDS or 1M urea)
Q8 – Is there a size limit for GFP labeled structures that can bind to the beads of the GFP-Trap?
Q9 – What should I do if there is residual background in my eluates?
We recommend to use our blocked particles (bab-20 or bmp-20) to pre-clear your samples.
Q10 – Which GFP variants does the GFP-Trap/ GFP-Booster recognize?
GFP, eGFP, wtGFP, GFP S65T, TagGFP, AcGFP, eYFP, YFP, Venus, eCitrine, Citrin, CFP
Not recognized: TurboGFP, all RFPs
Q11 – Which RFP variants does the RFP-Trap/ RFP-Booster recognize?
mRFP, mCherry, mOrange, mPlum, mRFPruby, mKate2, tdTomato, Strawberry
Not recognized: DsRed (very weak), TagRFP, all GFPs
Q12 – What are the biophysical parameters of GFP-Trap?
Molecular weight: 14.2 kDa
Theoretical pI: 9.2
Extinction coefficient (@ 280nm): 27,000
Q13 – What are the biophysical parameters of RFP-Trap?
Molecular weight: 15.1 kDa
Theoretical pI: 9.2
Extinction coefficient (@ 280nm): 29,900
Q14 – I’ve done a pull-down experiment on cell lysate using GFP-Trap Agarose. When using the anti-GFP antibody GMA3H9, I detect 2 different bands via Western Blot. Is this to be expected?
Yes, it is common to see a second degraded form of GFP or GFP-fusion protein.
Q15 – Which matrices are available for GFP-Trap? A14
We offer GFP-Trap as Agarose, Magnetic Agarose, and uncoupled as purified protein. These are good for:
- Pulldowns/ Immunoprecipitations
- Mass spectrometry
- Enzyme activity measurements
- ChIP analysis
|agarose beads||magnetic agarose beads|
|Particle size||90 µm||40 µm|
|Binding capacity||12 µg/10 µl||8 µg/10 µl|
|Centrifuged up to||2500 g||800 g|
Please choose the matrix depending on your preferences.