As accurate as FACS counting
Are you looking for an automated cell counter at the pinnacle of accuracy? Many labs have turned to the ADAM MC as a solution that yields highly repeatable results, as with a flow cytometer, but at a fraction of the cost. Its technology is simple; it combines a precision microscope with an integrated fluorescence detection optical system and onboard analysis software. With disposable chip-based kits that use propidium iodide to stain the nucleus of target cells, the ADAM never worries about cell size, shape, or aggregation. Instead it uses fluorescence to specifically image the stained nuclei, and averages 22 separate reads for each sample totaling a 3 µl read volume. These improvements specifically address variation attributable to low sample reads (some competitor models only perform one read), low sampling volumes (most models read 1 µl or less), and inaccurate resolution of cells (often found when cells clump together). With R2 values over 0.99, the ADAM MC can directly replace manual cell counting using the propidium iodide technique. The results are amazing, and it’s possible to get CVs under 10% with most cell lines. The ADAM MC can work with a dizzying range of suspension cell types, as only nuclei are needed for detection, and it uses a very clever “detergent wash” technique to provide precise cell viability values. But don’t take our word for it—we invite you to request a demo and find out for yourself.
Even an undergrad can use it
Did we mention that the ADAM MC is designed to be incredibly easy to use? Its disposable chip set minimizes the risk of contamination, greatly simplifies work flow, and eliminates the need for calibration and regular cleaning. Its on-board analysis obviates an external computer and allows quick operation. Finally, the ADAM MC is small enough to comfortably sit on any bench top—even an undergrad’s.
Comparison of cell viabilities between the ADAM and flow cytometry. SK-OV, HeLa and NCI-H23 cells
were treated 100 and 300µM H2O2 for 3 hours and then analyzed by the ADAM and flow cytometry.
Values are given as ± SD of three experiments.
These results show that with propridium iodide-stained CHO cells the ADAM MC counts are nearly identical to manual counting using a C-Chip Disaposable Hemocytometer. In practice, greater variability is possible because a glass hemocytometer cover slip floats, leading to wider variation in counting volumes. Additionally, trypan blue and propidium iodide stain different components of the cells. Propidium iodide and trypan blue counts typically vary by less than 5%.
Typical results with actual cell samples will vary between 5% and 20% CV.
The ADAM is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. The Adam provides two kinds of 1-2. Technology – Viability Measurement staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the Adam-MC software after each measurement of the total cells and the non-viable cells.
ADAM MC Auto Cell Counter
|Light Source||Green LED (532nm)|
|Dynamic Range||5 x 104 – 4 x 106 cells/ml|
|Analysis Time||45s – 1.5 min/test|
|Sample Volume||12 µl /test|
|Measuring Volume||3 µl/test|
|Tests Per Chip||400 – Cell viability
800 – Total cell count
|Cell Type||Suspension cells|
|Cell Size||Any size|
|Part Numbers||DBA01, DBA01K, ADR-1000|
|Detection Method||Stained cell nucleus|
|Power Requirements||100-240 VAC, 50-60 Hz|
|Size||220 x 375 x 250 mm|
|Warranty||1 year parts and labor|