BioReady Pfu DNA Polymerase

Highest Fidelity PCR

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Evolution delivers PCR with fewer errors

Pfu DNA polymerase is a thermostable DNA polymerase isolated from Pyrococcus furiosus. This well-characterized enzyme was the first to be shown to exhibit 3’ → 5’ exonuclease activity – necessary for proof reading. The result are PCR products with much lower error rates as compared with taq-based polymerases. The improved fidelity makes BioReady Pfu a great choice for certain applications including: template production for DNA sequencing, cloning and site-directed mutagenesis.

It ain’t rocket science any more

PCR used to be considered an art, but in the 30 years since its general acceptance into scientific experimentation it’s become one of the best-characterized bio-enzymatic processes. To this end, we ask you this simple question: why pay prices that don’t reflect this fact? All of Bulldog Bio’s PCR products are priced fairly and promise satisfying results. It’s that simple.

Species of Orgin pyrococcus furiosus
Enzyme Type Thermostable DNA polymerase
Part Numbers BRPPFU025, BRPPFU010, BRPPFU002
5’–3′ Exonuclease Activity Yes
“A” Overhang No, blunt end
3’–5′ Exonuclease Activity Yes
Reaction Buffer (included) 10x with 20mM MgSO4
Loading Dye (included) 6x
Storage 1 year at -20°C
Concentration 5 units/µl
Contains dNTPs No
DNase Free Yes
RNase Free Yes
Protease Free Yes
SYBR Green* Compatible Yes