Easy and rapid extraction from clinically derived samples
The Biospin Virus DNA/RNA Extraction Kit is designed for rapid isolation of DNA or RNA from most viral pathogens, including coronaviruses. Able to work with different starting sample sources including, fecal matter, swabs, fresh or frozen plasma, serum, other cell-free body fluids (urine, saliva etc.), and virus-infected cultured cells/tissue — this kit purifies nucleic acids that are free from contaminants and impurities, and are ideal for PCR and RT-PCR.
The simple, micro-spin column-based protocol can be completed in under 30 minutes. Relying upon Proteinase K digestion during lysis, this kit will degrade viral envelopes and the nucleocapsids. Nucleic acid molecules are released from these pathogens while RNAses and DNAses are inactivated by the same Proteinase K-containing lysis buffer. Nucleic acids are efficiently bound to a silica-gel membrane for rapid and effective purification of these nucleic acids without phenol extraction or ethanol precipitation.
Biospin Virus DNA/RNA Extraction Kit does not use harmful organic solvents, so it can be used safely many lab and testing environments. The genetic material purified by this kit can be used for pathogen detection, NGS analysis, cDNA synthesis, and other types of infectious disease research.
Biospin Virus DNA/RNA Extraction Kits
|Part Number||BSV050, BSV100|
|Number of Preps||50 or 100|
|Contents||Lysis buffer, proteinase K, wash buffer I, wash buffer II, elution buffer, micro-spin columns|
|Nucleic Acid Type||RNA & DNA|
|Sensitivity||DNA = 10 IU/mL; RNA = 100 IU/mL|
|Sample Types||Swab, fecal, supernatant of tissue homogenate, whole blood, serum, plasma, ascites, saliva and other liquid samples|
|Proteinase K Treatment||Yes|
|Final Elution Volume||50 μL – 100 μL|
|Storage||1 year at RT|