Bulldog HT Centrifugal Filtration Plates

Patented design offers simple, worry-free filtration of cells
The new HT Centrifugal Filtration Plates are compatible with both 96- and 384-well microplates and made to filter cells. Whether removing clumped debris from single cells preparations (such as for FACS or cell sorting), or collecting exosomes/proteins from the supernatant of a population of cells, HT Centrifugal Filtration Plates provide an answer. Save time, save money and prevent cross-contamination!

The plates solve many of the issues related to filtering cells by incorporating two patented technologies that can’t be found elsewhere.  The first improvement uses a silicone layer to tightly hold both plates and avoid inter-well contamination. The second technology uses a proprietary laser processing technology (PET) to attach a support membrane with an 300µm pore size for unmatched strength.  Combine this with four different standard pores sizes (0.45, 30, 70 & 100µm) of highly homogenous mesh (or track etched filter for 0.45µm), and these plates are perfect for cell filtration, collecting proteins, vesicles, exosomes, or many other applications.  Combine these features with the per-sample cost savings vs conventional cell strainers, and your lab lab can save money and time.

Did we mention these filtration are completely automation friendly? They are, and we can support use on most robotic systems.

Case study – No-cross contamination after plate centrifugation
The HT Centrifugal Filtration Plate was tested on a conventional 96-well round bottom plate for the presence or absence of contaminating cells after plate centrifugation. The two cell types tested included HEK293T not expressing GFP and HEK293T expressing GFP (human fetal kidney epithelial cell) were filtered in adjacent wells and measured with flow cytometry.

Results demonstrate that cell types remained in their corresponding wells with no observable cross-contamination. Data was collected from each well using a Guava EasyCyte 8HT and summarized below.

After centrifugation at 300g x 30 seconds, there was no contamination in another well and all the suspension had fallen to the bottom.