One-tube extraction from clinical samples
The Clean Viral DNA & RNA Kit allows rapid and reliable isolation of total nucleic acid from whole blood, serum, plasma, saliva, and other body fluids. Its hallmarks are a low investment of labor, time, and money to achieve equal or better results as compared to more traditional methods, such as spin columns.
The kit will extract Coronavirus (SARS-CoV-2 / COVID-19) RNA from clinically relevant samples including: i) nasopharyngeal swabs, ii) nasopharyngeal aspirates, and iii) bronchoalveolar lavage (BAL) samples. The Clean Viral DNA & RNA Kit combines a proprietary buffer system with the convenience of CleanNA magnetic particles to minimize the binding of PCR inhibiting compounds, present within the samples, onto the special magnetic particles.
Using the specially formulated lysis buffer made just for clinical samples containing viruses, such samples are lysed and the nucleic acid is bound to CleanNA’s engineered magnetic particles. During the lysis step DNases and RNases are immediately deactivated offering no chance for significant degradation of target nucleic acid molecules. RNA & DNA is isolated from the lysates in one step by binding to the surface of the proprietary magnetic particles. These magnetic particles are then separated from the lysates by using magnetic separation. Following a few rapid wash steps to remove trace contaminants (e.g. proteins and cellular debris), the purified DNA and RNA is eluted from the CleanNA particles using nuclease-free water or a low ionic strength buffer. No step during this entire protocol requires centrifugation or a temperature incubation. The isolated nucleic acid is directly suitable for use in most downstream applications, such as qPCR, NGS, and other molecular biology assays.
At no point during the protocol are samples needed to be centrifuged or incubated at temperatures other than room temperature. This is done by design and makes processing simple and automation-friendly. For labs with larger numbers of samples, or those that require higher levels of sample control, the kit protocol is fully scalable and can be used manually or can be easily automated on liquid handling workstations. A list of some systems validated for work with CleanNA products include:
- Dynamic Devices LYNX™
- Hamilton STAR™
- Biomek™ i5
- Biomek™ i7
- Thermo KingFisher™ Flex
- Applied Biosystems® MagMAX™
- Qiagen BioSprint
Expect equal or better results for RT-qPCR
The Clean Viral DNA & RNA Kit compare well against existing commercial products for the isolation and purification of viral RNA & DNA. The highly optimized magnetic bead and buffer technology all but ensures equal or better results as compared with existing purification protocols.
–Because of high demand only larger quantities are available – we cannot offer smaller samples–
Clean Viral DNA/RNA Kits
|Number of Preps||2304 or 24x 96-well plates|
|Contents||Lysis buffer, proteinase K, wash buffers, CleanNA beads, nuclease-free water|
|Nucleic Acid Type||RNA & DNA|
|Sensitivity||DNA = 5 IU/mL; RNA = 5 IU/mL|
|Sample Types||Whole blood, serum, plasma, saliva, and other body fluids, including nasopharyngeal SWABS, nasopharyngeal aspirates and bronchoalveolar lavage samples (BAL) samples|
|Proteinase K Treatment||Yes|
|Final Elution Volume||20 μL – 100 μL|
|Storage||1 year at RT|