CleanPCR Carboxylated Paramagnetic Beads

High recovery PCR clean-up reagent

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The purest amplicon DNA every time
The CleanPCR kit is a PCR clean-up reagent based on CleanNA’s carboxylated paramagnetic bead technology. These beads provide efficient purification of PCR amplicons over 100 bp in size. With its simple, three-step protocol, CleanPCR removes salts, as well as unincorporated primers, primer-dimers, and dNTPs. DNA fragments remain selectively bound to the carboxylated bead until eluted with low-salt elution buffer (or water). The highly purified DNA can be used directly for downstream applications. The protocol can be easily adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol. Or perform the simple protocol manually and get the same great results.

Why choose CleanNA carboxylated beads?

Carboxylated paramagnetic beads are the gold standard for removing impurities from dirty DNA or RNA preps. The technology, known as solid-phase reversible immobilization, is well-proven and very flexible across a multitude of applications. In a nutshell, when carboxylated beads resuspended in their binding buffer are added to an impure sample of nucleic acid (such as DNA) in the presence of alcohol, the beads “bind” the DNA in a jacket of water associated with the negatively charged carboxyl groups that coat the beads. After separation of the carboxylated beads from the solution using a magnet, the supernatant can be removed and the beads can be washed using alcohol, keeping the DNA trapped inside the jacket of water. This allows for a highly efficient impurity removal from the DNA. When the alcohol is removed and replaced with water, the trapped DNA is released very quickly and effectively. This efficient bind-and-release of DNA is the hallmark of carboxylated beads.

Application Purify amplified DNA from PCR sample
Part Numbers CPCR-005, CPCR-050, CPCR-500
Bead Type Carboxylated & Paramagnetic
Separation Technology Solid Phase Reversible Immobilization
Number Purifications 5ml purifies 138 x 20 ul PCR reactions
Size Selection Amplicons greater than 100bp
Removes Unincorporated dNTPs, primers, primer dimers, and all other contaminants
Filtration required No
Centrifugation required No
Storage 1 year at 4°C to 8° (do not freeze)