Perfect square wave pulses
The act of delivering DNA or RNA into living tissue by electrical pulsing has been a standard lab practice for over two decades. Typically, specialized electrodes are used to target the exact delivery region. Protocols and pulsing parameters can vary greatly, but it’s acknowledged across the board that systems using square-wave electrical pulses provide better and more consistent results. The CUY21 EDIT is just such a system, designed to deliver up to 99 square wave pulses that can be set between 1V and 500V. Most in vivo experiments require applying pulses under 100V to help preserve tissue integrity. For some electroporators, it can be challenging to hold such low voltages accurately and with a perfect squared form–not so for the CUY21 EDIT. With such precise control, it’s no surprise that this system has been used in over 500 published research articles.
Knowing electrical impedance is imperative
The electrical conditions for in vivoexperiments will vary from day to day and from sample to sample. Impedance (a measure of electrical resistance) is the single most critical parameter to maintain in these types of experiments. The easiest way to standardize this parameter, and thus to maintain consistent results, is to fine-tune the Impedance to the exact range that you desire. The CUY21 EDIT makes this easy by allowing you to measure Impedance BEFORE you proceed with the pulsing. This affords you the opportunity to adjust the setup (typically distance between electrodes, buffer composition, or buffer volume) to ensure a successful transfection without risking your precious cells. The system will also provide actual electrical output (V, A, and Ohms) after each pulsing step in real time, so you can ensure that there are no issues as you go!
In vivo experiments use a huge variety of electrodes, but what all of these wires, paddles, and needles share is the potential to deliver a nasty shock to an unwary grad student or research assistant. The CUY21 EDIT is equipped with an integrated safety limit that will immediately shut down the flow of electricity if excess current is drawn.
Click here to see available electrodes for in Vivo, in Utero, in Ovo and ex Vivo applications.
CUY21 EDIT In Vivo Transfection System
|DC pulse waveform||square|
|Voltage||1-500V (1V increments)|
|Pulse Length||0.1-999.9 ms|
|Pulse Interval||0.1-999.9 ms|
|Number of pulses||1-99|
|Power Requirements||115V or 220V, 50/60Hz|
|Size||390(W) x 450(D) x 250(H) mm|