“In Processes Monitoring” for Surefire Results
The DNA-spin Plasmid DNA Purification Kit is a simple two-step process – break open walled cells and purify plasmid DNA. Based on the proven technology of optimized alkali lysis buffering system and silica membrane, this kit will isolate extremely pure plasmid DNA from bacteria. The kit also includes a neutralization buffering technique that is applied for gentle pH conditions during binding. This not only stabilizes the isolation and purification, but also efficiently removes chromosomal DNA and cell debris…particularly for plasmids larger than 30 Kb. Additionally, our novel “Lysis Viewer” is added to the reaction for a visual pH confirmation. Different colors indicate proper lysis and neutralization, so you can easily see the steps as they occur. The DNA-spin Plasmid DNA Purification Kit can be used for both high-copy and low-copy plasmids so it is perfect for all your small-scale plasmid purification needs (up to 35µg).
For even faster and more efficient plasmid purfication (about 10-minutes witha 20-30% yield increase) see our new Fast DNA-spin Plasmid Purificaiton Kits.
Plasmid DNA was extracted from 5 ml (OD600) of E. coli cultures containing 2.9 Kb, 6.5 Kb, 8 Kb, 12.5 Kb and 33.4 Kb plasmid DNA, respectively. Purification yield and purity were analyzed. 1 mL was analyed on Nano-drop ND-2000 UV/VIS.
DNA-Spin Plasmid Purfication Kit
200 Column Kit
|Resuspension Buffer||55 mL|
|Lysis Buffer||55 mL|
|Neutralization Buffer||80 mL|
|Washing Buffer A||140 mL|
|Washing Buffer B||40 mL|
|Elution Buffer||20 mL|
|Spin Columns w/ cap||200 tubes|
|Collection Tubes||200 tubes|
|RNAase A, Lyophilized||35 mg|
|Lysis Viewer||220 µL|
|Shelf Life||2 Years|