PCR done the easy way
Are you tired of the time-consuming extraction processes and costly spin columns that you’ve been using to prepare samples for DNA amplification? With the DNAreleasy Advance Direct Lysis Kit, we now have a better solution. This new cell lysing reagent requires only a 15 minute incubation in a thermal cycler before the DNA is ready for use directly in your PCR—without any further sample processing! DNAreleasy Advance has been successfully used to lyse a wide range of cells. These include saliva, hair roots, animal tissue, plants, drosophila, yeast, bacteria, viruses and many more.
Using DNAreleasy Advance is real easy (no pun intended). Just mix cells with 20 μl of the reagent, place in a a thermal cycler or incubator and heat at 75°C for 5 minutes, followed by 96°C for 5 minutes before holding at 20°C for 5 minutes. After the lysis, a part or all of the lysate can be added directly to your PCR mix or it can be stored at -20°C for future use.
Genomic DNA from scallops was isolated with DNAreleasy Advance, and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield obtained.
The same amplified DNA from scallops was used for sequencing, resulting in high-quality reads.
Total DNA was isolated from different plant species using DNAreleasy Advance. Melting curves were determined by RT PCR:
Positive control, maize meal, wheat flour, sugar beet, white cabbage, negative control.
Genomic DNA was isolated using DNAreleasy Advance and analyzed by qPCR:
(1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melanogaster, (6) horse meat
DNAreleasy Advance , LS06
|Amount used in PCR||Up to 10%|
|SYBR Green Compatible||Yes|
|Cell and Tissue Compatible||Yes|
|Lysate Can Frozen for Use Later||Yes, at -20°C|
|Storage||1 year at -20°C|