You choose the buffer and pulse settings
Our partners at Nepa Gene are pioneers in multi-phase electroporation. First, their CUY21 EDIT offered 2-phase pulse control. Then their NEPA21 bumped that up to 4-phase pulse control. The NEPA21 has proven to be superior to any other electroporator on the market – capable of transfecting primary cells, cell lines, adherent cells, embryos, tissue explants, and even whole organisms! The only life form that proved elusive were small walled cells, such as yeast and bacteria. Now the ELEPO21 has torn this wall down, too. Coupling high precision 4-phase voltage control with a greatly expanded range of voltages, the ELEPO21 can juice your hardiest cells with up to 3,000V of pure electrical energy! Results using the ELEPO21 on E. coli and S. cerevisiae bear out its remarkable advantages. WITHOUT using expensive, proprietary buffers, the ELEPO21 has shown an average of approximately 5x increase in transformation efficiency as compared with simple single-pulse electroporators. This instrument works with all types of suspension cells, including common mammalian cell lines, primary cells, and plant cells. Read more about the secrets behind Nepa Gene’s proprietary pulsing technology below.
Above are 3 different pulsing conditions on the ELEPO21 compared with previously optimized conditions on a MicroPulserTM. The Poring Pulse conditions represented from left to right for the ELEPO21 are 1250 V for 3.5 ms, 1250 V for 5 ms, and 1500 V for 3.5 ms. The MicroPulserTM also delivered a single pulse at 1500V for 5 ms. All ELEPO21 conditions also included a Transfer Pulse phase consisting of 3 pulses at 100 V for 50 ms. Data provided by Nepa Gene courtesy of Dr. Yasuhori, Chiba Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST).
The ELEPO21 in Vitro Electroporation System’s 4-step pulse with voltage decay results in higher transfetion efficiency and lower damage, without the use of special buffers.
Step 1 – Poring Pulse: High voltage, short duration, multiple pulses, voltage decay.
The poring pulse is for forming small holes in the cell membrane with minimal damage.
Step 2- Poring Pulse Reverse Polarity: This increases efficiency with adherent cells and other types of transfections.
Step 3 – Transfer Pulse: Low voltage, long duration, multiple pulses, voltage decay.
The transfer pulse is for delivering the target molecules (DNA, RNA, etc.). Like low voltage electrophoresis, these extra-long pulses carry charged molecules into cells with minimal damage.
Step 2- Transfer Pulse Reverse Polarity: This increases efficiency with suspension cells, adherent cells, and other types of transfections by 10-30%.
ELEPO21 In Vitro High Voltage Electroporator
|# pulsing phases||Up to 4|
|Types of pulses||Poring and Transfer|
|# of poring pulses||0-9|
|# of transfer pulses||0-20|
|Total energy readout||Yes, in Joules|
|Size||432(W) x 336(D) x136(H) mm|