Endo F3 PRIME-LY
Cleaves in β(1-4) Link Between the Two Core GlcNAcs of Asparagine Linked Glycans
Made for core fucosylated N-glycan analysis
Endo-β-N-acetylglucosaminidase F3 (Endo F3) cleaves in β(1-4) link in between the two core GlcNAcs of asparagine linked glycans. Endo F3 cleaves this link on core-fucosylated structures. Endo F3 can be applied to workflows alone or in conjunction with PNGase F PRIME to allow for structural characterization of core-fucosylated glycans in tissues while maintaining spatial localization. Endo F3 PRIME-LY™ comes in a lyophilized format that is perfectly suitable for use in solution-based analyses.
For instance, one major drawback of MALDI Imaging is the inability to differentiate core-fucosylated glycans from outer-arm fucosylated glycans. This issue is alleviated through by using Endo F3 PRIME-LY due to its specificity for core-fucosylated structures.
Core Fucosylated N-Glycan Analysis in FFPE Tissues
While increased levels of fucosylation have been previously seen in hepatocellular carcinoma, one major drawback of MALDI Imaging is the inability to differentiate core-fucosylated glycans from outer-arm fucosylated glycans. This issue is alleviated through by using Endo F3 PRIME-LY™ due to its specificity for core-fucosylated structures. Pairing Endo F3 PRIME-LY™ with traditional PNGase F PRIME-LY™ imaging allows for the differentiation between core-fucosylated and outer-arm fucosylated glycan structures, allowing for the further classification of fucosylated glycans upregulated in cancer specific regions and giving a more definitive and specific alteration patter to disease states.
Serum/Plasma N-Glycan Profiling of Fucose Isomers
Application of both PNGase F PRIME-LY™ and Endo F3 PRIME-LY™ for N-glycan release were utilized in a recently developed method for N-glycan profiling of whole serum and plasma. Using this method, over 75 N-glycan species can be detected from one microliter of serum in less than 6.5 hours, demonstrating the potential applications of this method in clinical diagnostics. The ability to utilize both PNGase F PRIME-LY™ and Endo F3 PRIME-LY™ in this method represents the feasibility of differentiating fucose isomers within this method.
Endo F3PRIME LY - Lyophillized
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SPECIFICATIONS
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| Part Numbers | NZEF3010LY |
| Target | alpha-1,6 linked fucosylated Asparagine-linked (N-linked) oligosaccharides (complex) |
| Compatible Glycoprotein | Native an denatured |
| Biological Source | Recombinant, E. coli |
| Total Protocol Length | 30 minutes to 24 hours |
| Experiment Types | in vitro and in situ |
| Typical Yield | 1 Unit of enzyme will completely deglycosylate 10 µg of denatured IgG following incubation for 10 minutes at 37°C. 1 Unit = 1 IUB milliunit. |
| Concentration | 8 Units/µL after resuspending in 100µL of dH2O. |
| Purity | ≥95% as determined by SDS PAGE |
| Compatible with Mass Spec | Yes |
| Contains Glycerol | No |
| Affinity Tag for Purification | Yes. Endo F3 PRIME contains a His-tag. |
| Storage | Supplied lyophilized and shipped at ambient temperature. Once reconstituted, store at -20°C or 4°C (avoid multiple freeze-thaws) |
| Shelf Life | 1 year |
| Microbe Free | Yes, lot tested |