Image the finest details of DNA & RNA gels
The FAS-Digi PRO Gel Imaging system is for sensitive and safe documentation of fluorescently-stained nucleic acid in gels. This imaging platform is built around the new FastGene Blue/Green LED Gel TransIlluminator XL which utilizes the superior technology of ultra-bright blue/green LEDs. The aquamarine-color illumination hits the sweet spot for exciting common fluorescence DNA dyes such as EtBr and Sybr Green. You can always expect at least equivalent results as compared to UV transilluminators, but without the risk of damaging DNA or harming your skin and eyes. That’s because the lower energy photons from these LEDs, unlike UV light, will not cross-link DNA (which reduces its subcloning efficiency). The system is designed to produce stronger signals and lower background by illuminating from opposite sides (dual LED matrices) as opposed to directly shining light from below like, most other gel illuminators. The FAS-Digi PRO is developed for use with either red dyes such as ethidium bromide (EtBr) or next generation non-carcinogenic, non-toxic dyes like our Midori Green family of safe dyes. LEDs are safer and more efficient than traditional UV bulbs making the FAS-Digi PRO system very compact and extraordinarily durable.
Performance specifications that make your results better
The system is designed to produce stronger signals and lower background! The huge imaging area is 26 cm x 21 cm and has unsurpassed uniformity of lighting. 12 LED arrays illuminate from opposite sides as compared to directly shining light from below like other gel illuminators. The FAS-Digi PRO was developed for use with either red dyes such as ethidium bromide (EtBr) or next generation non-carcinogenic dyes like our Midori Green or Sybr Green-based dyes.
Equipped with an über-light-sensitive 24 MPixel camera, the FAS-Digi PRO is controlled completely by our newly developed imaging software. With the “live view” mode, all changes to the camera, the exposure time, the lens aperture, and a digital zoom are displayed in real-time. It’s a fully networkable gel documentation system, which enables easy transfer of images when connected to a PC.
The absorbance spectrum of Ethidium Bromide, Midori Green Advance, and Midori Green Direct
Upgrade Your Camera
Take images of agarose gels of the highest quality using the Canon EOS 200D. This camera has an immense APS-C CMOS-sensor. The sensor produces no visible noise at all from ISO 100 all the way up to ISO 1600. Furthermore, the 24 megapixels allows the detection of even the faintest light signals. The exposure time of the sensor can be set from 1/4000 sec up to 30 sec. A very short exposure guarantees that even the brightest DNA band will be visible within the dynamic range. Exposing the sensor for up 30 sec will enable the detection of bands that your eyes cannot see. The 3x zoom (focal length of 18 mm to 55 mm) allows a perfect enlargement of the area of interest.
Nifty onboard software
The FastGene FAS-Digi PRO comes with the intuitive NIPPON Genetics Camera Studio software. With this software you can control all necessary parameters of the camera to analyze and optimize any gel image. These four settings will provide the highest quality images your lab has ever seen for DNA gels: aperture, exposing time, sensitivity and focus. Mouse-driven optimization makes image optimization a click away! Images are saved as TIFF and JPEG format, and can printed directly by a printer connected to your PC. Need higher quality prints? We offer the Mitsubishi Thermal Printer (P95DE) which creates brilliant prints on high-glossy paper.
Get your DNA the easy way
Safe Illumination vs Traditional Illumination
With the FastGene FAS-Digi PRO Imager and Midori Green Stain, it is so simple to cut any desired DNA fragment out of gels. You don’t need to wear protective eyewear, or worry about mutagenic dyes. Remove the shroud, switch on the LEDs and excise your DNA fragment with our FastGene Agarose Gel Band Cutter. Fast, precise and totally safe, it’s the 21st century way of working with DNA. There is simply no reason to put yourself or your lab staff at risk during routine handling of DNA for subcloning, genetic analysis or other applications.
Safer for you and better for your subcloning
By using safe DNA dyes and safe LED illuminators you can improve your subcloning transformation efficiencies by THREE-FOLD. In this example, a plasmid vector was double digested with suitable restriction enzymes to create two sticky-ended DNA fragments: the lacZ gene (3,536 bp) and the backbone of the vector (4,318 bp). Equal amounts of digested DNA were electrophoresed on 1% agarose gels. The gels were stained with either ethidium bromide or Midori Green Direct gel stain according to the corresponding manuals, and then viewed using either a UV transilluminator or the FastGene Blue/Green LED Illuminator, respectively. The two DNA fragments were excised from the gels and purified using a silica membrane based purification kit. The lacZ gene and the vector backbone were religated using T4 DNA ligase transformed into DH5a cells and plated onto selection plates. The total number of blue and white colonies was counted to evaluate cloning efficiency. Each experiment was conducted in triplicate, and the average cloning efficiency was determined. Midori Green Direct resulted in dramatic increase of positive transformants.
Midori Green Can Boost Your Cloning Results!
Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short wave-length light is well known to cause thymidine dimers and damage the DNA. The extent of this damage is not always appreciated. High energy light wreaks havoc on a DNA fragment in mere seconds. As can be seen below, after only a 15 sec exposure of DNA in a standard agarose gel, cloning efficiency starts to drop. And after a 30 sec exposure your cloning experiment is all but dead! In contrast, the cloning efficiency of protocols that use Blue LEDs seem completely immune to any deleterious effects. If your lab isn’t able to break itself of its ethidium bromide habit, using a Blue/Green LED illuminator (and imaging systems) have no significant impact on cloning efficiency or DNA integrity.
UV Transilluminators Kill Cloning Experiments
FAS Digi PRO LED Imaging System
FAS Digi PRO Accesories
-Green fluorescent dyes such as Sybr Green, Midori Green & GelGreen, ext.
-Red fluorescent dyes such as EtBr & GelRed, ext.
PROTEIN GELS & BLOTS
– SYPRO Ruby, Coomassie
– Colormetric blots
PETRI DISH documenation
|Filter||MIDORI Green Amber filter|
|LED Array||Dual matrices for two-sided illumination|
|LED Lifetime||50,000 hours|
|Lens||18-55 mm zoom Lens, manual, F/4-5.6|
|Camera||Canon 200D, 24 MPixel (Resolution: 6000×4000), APS-C Sensor DIGIC 8 Processor, WiFi enabled|
|Exposure time||0.00025 to 30 seconds|
|Control Software||Included for PC – NIPPON Genetics Camera Studio v1.0, WINDOWS 10|
|Size||33.5 x 52 x 32.5 cm (length x height x depth)|
|Illumination Surface||26 x 21cm|
|Image Type||TIFF, JPEG|