The first portable gel imaging system
The FastGene FAS Nano LED System is the most compact gel illumination system on the market. Ideally suited for tight spaces on a bench-top, the system operates both as an illuminator and, if equipped with a smart phone or tablet, a documentation system that captures an image of your gel.
An array of blue/green LEDs situated around the periphery of the glass platen provide excitation light for both red- and green–emitting fluorescence dyes. Now you can get great images with traditional ethidium bromide (EtBr), not previously possible with older-style blue LED illuminators. This optimal “transillumination” configuration minimizes ambient background for the most sensitive imaging with non-carcinogenic dyes like Midori Green, or other green dyes such as Sybr Green. Safer than old-style UV transilluminators, the blue/green LEDs are just as sensitive as UV light but they don’t damage DNA in an agarose gel and they won’t harm your skin or your eyes.
The absorbance spectrum of Ethidium Bromide, Midori Green Advance, and Midori Green Direct
Discover the FastGene FAS-Nano Gel Illumination System
Safe Illumination vs Traditional Ilumination
Get your DNA the easy way
With the Fastgene system and Midori Green Stain it becomes extremely simple to excise your DNA fragments from gels. You don’t need to wear protective eyewear or worry about mutagenic dyes—just switch on the blue-green LEDs and excise your DNA fragment with our Fastgene Agarose Gel Band Cutter! Fast, precise, and totally safe, it’s the twenty-first-century way of working with DNA.
Safer for you and better for your subcloning
By using safe DNA dyes and safe LED illuminators you can improve your subcloning transformation efficiencies by THREE-FOLD. In this example, a plasmid vector was double digested with suitable restriction enzymes to create two sticky-ended DNA fragments: the lacZ gene (3,536 bp) and the backbone of the vector (4,318 bp). Equal amounts of digested DNA were electrophoresed on 1% agarose gels. The gels were stained with either ethidium bromide or Midori Green Direct gel stain according to the corresponding manuals, and then viewed using either a UV transilluminator or the FastGene Blue/Green LED Illuminator, respectively. The two DNA fragments were excised from the gels and purified using a silica membrane based purification kit. The lacZ gene and the vector backbone were religated using T4 DNA ligase transformed into DH5a cells and plated onto selection plates. The total number of blue and white colonies was counted to evaluate cloning efficiency. Each experiment was conducted in triplicate, and the average cloning efficiency was determined. Midori Green Direct resulted in dramatic increase of positive transformants.
Midori Green Can Boost Your Cloning Results!
Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short wave-length light is well known to cause thymidine dimers and damage the DNA. The extent of this damage is not always appreciated. High energy light wreaks havoc on a DNA fragment in mere seconds. As can be seen below, after only a 15 sec exposure of DNA in a standard agarose gel, cloning efficiency starts to drop. And after a 30 sec exposure your cloning experiment is all but dead! In contrast, the cloning efficiency of protocols that use Blue LEDs seem completely immune to any deleterious effects. If your lab isn’t able to break itself of its ethidium bromide habit, using a Blue/Green LED illuminator (and imaging systems) have no significant impact on cloning efficiency or DNA integrity.
UV Transilluminators Kill Cloning Experiments
FAS-Nano Gel Illumination System
-Green fluorescent dyes such as Sybr Green, Midori Green & GelGreen, ext.
-Red fluorescent dyes such as EtBr & GelRed, ext.
|Emission Filter||540nm long pass Amber fliter & amber shield|
|Wavelength||470 – 520 nm|
|LED Lifetime||50,000 hours|
|Camera Compatibility||Smart phone or tablet|
|Imaging Area||10cm x 10cm|
|Size||21.6 x 16.8 x 12.8 cm (Length x Width x Height)|
|Power||12V, 1.25A, 100-240V, 50-60Hz|