FastGene PROBE One Step Mix – Separate ROX

Rapid Detection using Multiplex qPCR from RNA

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Great buffer, great enzyme, great results!
FastGene PROBE One Step Mixes were developed for the rapid detection of multiple expressed genes using multiplex qPCR directly from RNA. PROBE One Step mixes are compatible with fluorophore/quencher probe-style assays that are detected by 5´to 3´ exonuclease activity and can replace any commercial probe-based qPCR mixture.

Both the enzyme and the buffer were specifically optimized for remarkable efficiency – ensuring near-perfect performance for both the reverse transcription and the amplification reaction phases. Your lab can expect these kits to deliver high sensitivity so that even low copy genes are detected. From low-copy to high-copy RNA targets, reproducible results are the rule with FastGene PROBE One Step Mixes – even for GC- and AT-rich templates. The proprietary buffer enables faster reaction times (including a 10 min RT step) allowing more runs in a typical workday, and without the worry of variable results.

Use as either high ROX (500 nM) or low ROX (50 nM) with included separate ROX  stock solution.  The FastGene PROBE One Step Mix is compatible with nearly every qPCR instrument on the market. Here are some common experiments that can be performed with FastGene PROBE One Step Mixes:

  • Quantification of gene expression
  • Quantification of gene copy number
  • Detection of viral RNA
  • Multiplex qPCR
  • SNP genotyping
  • NGS validation

Part Number LS4701SR, LS4705SR
Number of Reactions 100 or 500
Reaction Volume 20 µL
Contents 1 mL readymix or 5x 1 mL readymix
Template RNA
Fluorophore ROX (separate tube for high or low concentration)
PROBE Reaction Type FastGene Probe 1-Step RT-qPCR Kit can replace
any commercial Probe based qPCR mixture. The
annealing temperature may need to be optimized
Storage 1 year at -20 °C and for at least 30 freeze thaw cycles