FastGene RNA Premium Purification Kit

For Ultrapure & Highly Concentrated RNA

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New level of RNA purity
The FastGene Premium RNA kit was developed for rapid, efficient, and clean purification of RNA from tissues and cells for challenging applications such as Next Gen sequencing (NGS). The kit’s silica-based technology dispenses with phenols in favor of superior DNase treatment to ensure the near-complete elimination of genomic DNA. It is the only RNA purification kit available that uses three separate column technologies. This results in extracted RNA of the highest concentration and purity – free from contaminating nucleic acids, proteins and other bio-molecules.


RNA of the highest quality
The FastGene Premium RNA kit was developed for rapid, efficient, and clean purification of RNA from tissues and cells for challenging applications such as Next Gen sequencing (NGS). The kit’s silica-based technology dispenses with phenols in favor of superior DNase treatment to ensure the near-complete elimination of genomic DNA. It is the only RNA purification kit available that uses three separate column technologies. This results in extracted RNA of the highest concentration and purity – free from contaminating nucleic acids, proteins and other bio-molecules.

Fig. 1: RNA Quality determination using an Agilent Bioanalyzer. The FastGene Premium RNA Kit repeatedly delivers high quality RNA .


Large yields
Obtaining a good yield from the RNA purification is essential. The FastGene RNA Premium Kit is optimized to deliver very large total yields. More RNA enables more analyses from each RNA prep.

Fig. 2: Total Yield of nucleic acid in µg. The FastGene RNA Kits delivers very high yield.


Highest concentration and near-perfect recovery
The FastGene Mini Elute column is an important component of the kit. Its unique design allows elution volumes as low as 10 µl.  Getting highly concentrated RNA stocks from a small amount of tissue or cellular material is a remarkable feat. Moreover, the recovery rate is very high (>95 %) even at very low elution volumes. The FastGene Premium RNA Kit provides a unique combination of benefits – high concentrations, high yields, high purity, and high recovery rates. See the results for yourself below:


SPECIFICATIONS
Part Number NGFG81006 NGFG81050 NGFG81250
Number Sample Preps 6 50 250
Lysis Buffer 4 ml 25 ml 125 ml
Wash Buffer 1 4 ml 35 ml 170 ml
Wash Buffer 2 2 ml 20 ml 2 x 50 ml
RNA Re-Binding Buffer 1 ml 8 ml 36 ml
Elution buffer (RNase free water) 1.5 ml 30 ml 200 ml
DNase I reconstitution solution 1.5 ml 1.5 ml 1.5 ml
10 x DNase I reaction buffer 50 µl 500 µl 2 x 1 ml
DNase I (lyophilized) 110 Kunitz units 110 Kunitz units 560 Kunitz units
FastGene RNA filter column 6 50 250
FastGene RNA binding column 6 50 250
FastGene RNA mini-elute column 6 50 250
1.5 ml collection tubes 12 100 500
2 ml collection tubes 18 150 750
FastGene mini-elute column (neutral color) Storage 2-8°C
FastGene RNA Premium Kit Storage Room Temp
Shelf Life 1 Year